Augmentation and Repair of Vocal Cord Tissue Defects

• US 20080311089A1
• Filed: 08/19/2008
• Published: 12/18/2008
• Est. Priority Date: 02/20/1997
• Status: Active Grant

• Current Assignee: Castle Creek Biosciences LLC (Castle Creek Biosciences, Inc.)
• Original Assignee: Fibrocell Science, Inc.
• Inventors: Keller, Gregory S.
• Granted Patent: US 8,261,749 B2
• Time in Patent Office: Days
• US Class Current: 424/93.7
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3641 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61P 11/04 for throat disorders
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0656 Adult fibroblasts

Abstract

The present invention provides a method for corrective surgery in a human subject of a vocal cord defect being amenable to rectification by the augmentation of tissue subjacent to the vocal cord defect comprising the steps of:

• • a) retrieving a plurality of viable cells from the subject;
  • b) culturing the viable cells in vitro; and
  • c) placing an effective volume of the in vitro cultured cells into a tissue of the subject, the tissue being located in a position subjacent to the vocal cord defect to be rectified. The present invention further provides a method for corrective surgery in a human subject of a vocal cord defect being amenable to rectification by the augmentation of tissue subjacent to the vocal cord defect comprising the steps of:
  • a) retrieving a plurality of viable cells from the subject;
  • b) culturing the cells in vitro in a culture vessel for a time sufficient for the cells to produce extracellular matrix;
  • c) separating the extracellular matrix produced by the cells from the culture vessel;
  • d) collecting the extracellular matrix; and
  • e) placing the collected extracellular matrix into a tissue subjacent to the vocal cord defect.

Claims

1-20. -20. (canceled)

21. A method for corrective surgery in a human subject of a vocal cord defect amenable to rectification by the augmentation of tissue subadjacent to the vocal cord defect, the method comprising:

placing an effective amount of autologous in vitro cultured cells into a vocal cord tissue of the subject in a position subadjacent to the vocal cord defect, wherein the vocal cord tissue is selected from the group consisting of a scar, Reinke'"'"'s space, a muscle of the vocal cord, and the lamina propria, and wherein the in vitro cultured cells are obtained by culturing a plurality of viable cells retrieved from the subject.