Methods and Means for Diagnostics, Prevention and Treatment of Mycobacterium Infections and Tuberculosis Disease

• US 20080311159A1
• Filed: 03/31/2006
• Published: 12/18/2008
• Est. Priority Date: 03/31/2005
• Status: Abandoned Application

• Current Assignee: Leiden University Medical Center
• Original Assignee: Leiden University Medical Center
• Inventors: van Meijgaarden, Krista Elisabeth, Franken, Cornelus Leonardus Maria Coleta, Lin, Min Yong, Klein, Michel Robert, Ottenhof, Tom Henricus Maria, Leyten, Eliane Madeleine Sophie
• Application Number:
  US11/910,125
• Publication Number:
  US 20080311159A1
• Time in Patent Office: Days
• US Class Current: 424/248.1
• CPC Class Codes: A61K 39/04 Mycobacterium, e.g. Mycobacterium tuberculosis
• CPC Class Codes: A61P 31/06 for tuberculosis
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: G01N 33/5695 Mycobacteria

Abstract

The invention identifies a narrow subset of Mycobacterium latency associated antigens and or epitopes that are capable of eliciting an immune response in vivo and in vitro in a mammal. The invention provides methods and compositions for detection and immunization against latent Mycobacterium infections. These compositions comprise those Mycobacterial latency antigens that are actually capable of eliciting an immune response in vivo, in mammals experiencing a latent Mycobacterium infection. More preferably the compositions comprise those antigens that are preferentially recognized by latently infected individuals and which antigens are not, or to a much lesser extent, recognized in individuals having an active Mycobacterium infection or in individuals having Mycobacterium induced symptoms or diseases, such as in patients infected with M. tuberculosis suffering from tuberculosis disease (TB).

Claims

1. A method for inducing an immune response against a Mycobacterium infection in a vertebrate, the method comprising the step of administering to the vertebrate a composition comprising a source of one or more polypeptides selected from the group consisting of M. tuberculosis dormancy (DosR) regulon sequences:

Rv0079, Rv0569, Rv1733c, Rv1738, Rv1813c, Rv1996, Rv2007c, Rv2029c (PfkB), Rv2030c, Rv2031c (HspX), Rv2032, Rv2626c, Rv2627c, Rv2628, Rv3126c, Rv3129, Rv3130c, Rv3132c, Rv3133c (DosR), Rv0080, Rv1737c (NarX), Rv1735c and Rv1736c (NarK2), and analogues, homologues or fragments thereof.

2. The method of claim 1 wherein the polypeptide is selected from the group consisting of Rv1733c, Rv2029c (PfkB), Rv2627c, Rv2628, Rv0080, Rv1737c (NarK2), Rv1735c and Rv1736c (NarX).

3. The method of claim 2 wherein the polypeptide is selected from the group consisting of Rv1733c, Rv2029c and Rv2627c.

4. The method of claim 2 wherein the polypeptide is obtained from the TB dormancy regulon (DosR) sequences Rv1733c, Rv2029c (PfkB) and Rv0080.

19. A diagnostic kit for diagnosing Mycobacterium infections in a subject comprising one or more polypeptides of claim 1 and reagents to assay and quantify antibody binding to said polypeptides.

5. A composition for immunization against Mycobacterium infections comprising a source of one or more polypeptides selected from the group consisting of TB dormancy (DosR) regulon sequences:

Rv0079, Rv0569, Rv1733c, Rv1738, Rv1813c, Rv1996, Rv2007c, Rv2029c (PfkB), Rv2030c, Rv2031c (HspX), Rv2032, Rv2626c, Rv2627c, Rv2628, Rv3126c, Rv3129, Rv3130c, Rv3132c and Rv3133c (DosR), Rv0080, Rv1737c (NarX), Rv1735c and Rv1736c (NarK2), and analogues, homologues or fragments thereof, and optionally comprising an adjuvant.

6. The composition according to claim 5 wherein the polypeptide is selected from the group consisting of Rv1733c, Rv2029c (PfkB), Rv2627c, Rv2628, Rv0080, Rv1737c (NarK2), Rv1735c and Rv1736c (NarX).

7. The composition according to claim 6 wherein the polypeptide is selected from the group consisting of Rv1733c, Rv2029c and Rv2627c.

8. The composition according to claim 6 wherein the polypeptide is obtained from the TB dormancy regulon (DosR) sequences Rv1733c, Rv2029c (PfkB) and Rv0080.

9. The composition for immunization according to claim 5 wherein the adjuvant is selected from the group of adjuvants consisting of polyI:

C, CpG, LPS, lipid A and derivatives thereof, IC31, QS21, lipopeptide Pam3Cys, bacterial flagellins, DDA/MPL, DDA/TDB and soluble LAG3.

10. The composition for immunization according to claim 5 wherein the Mycobacterium polypeptide is selected from Mycobacterium TB complex species M. tuberculosis, M. bovis, M. bovis BCG, M. africanum, M. canetti and M. microti.

11. The composition for immunization according to claim 5 wherein the source of the polypeptide is a recombinant DNA molecule encoding the polypeptide, optionally comprised in a vector and/or in a genome.

12. The composition for immunization according to claim 5 wherein the source of the polypeptide is in a recombinant Mycobacterium, preferably Mycobacterium bovis Bacillus Calmette-Guerin (BCG).

13. The composition for immunization according to claim 5 wherein the polypeptide fragments are synthetic peptides, preferably between 18 and 45 amino acids in length, optionally overlapping or ligated, optionally containing additional amino acids, immuno-stimulating moieties and or protective groups to enhance solubility and increase stability in vivo.

14. The composition for immunization according to claim 5, further comprising a CD40 binding molecule selected from an antibody or fragment thereof or a CD40 ligand or a variant thereof.

15. The composition for immunization according to claim 5, further comprising an agonistic anti-4-1BB antibody or a fragment thereof, capable of stimulating the 4-1BB receptor.

16. The composition for immunization according to claim 5 wherein the composition further comprises a Mycobacterium antigen that is not specific for the latency stage.

17. A method for diagnosing Mycobacterium infections in a subject comprising the steps of:

a) contacting a sample of isolated bodily fluid and/or isolated white blood cells of the subject with one or more polypeptides or fragments thereof selected from the group of proteins encoded by the TB dormancy regulon consisting of;

Rv1733c, Rv2029c (PfkB), Rv2627c, Rv2628, Rv0080, Rv1737c (NarK2), Rv1735c and Rv1736c (NarX); and

,

b) detecting a humoral immune response by binding of antibodies to said polypeptide(s) or fragments thereof being indicative of Mycobacterium infection and/or;

c) detecting a cellular immune response by specific proliferation and/or cytokine production and/or expression of extra- or intracellular activation markers.

18. The method according to claim 17 wherein the polypeptides are selected from the group consisting of Rv1733c, Rv2029c, Rv2627c and Rv0080.