Methods and Means for Diagnostics, Prevention and Treatment of Mycobacterium Infections and Tuberculosis Disease
First Claim
1. A method for inducing an immune response against a Mycobacterium infection in a vertebrate, the method comprising the step of administering to the vertebrate a composition comprising a source of one or more polypeptides selected from the group consisting of M. tuberculosis dormancy (DosR) regulon sequences:
- Rv0079, Rv0569, Rv1733c, Rv1738, Rv1813c, Rv1996, Rv2007c, Rv2029c (PfkB), Rv2030c, Rv2031c (HspX), Rv2032, Rv2626c, Rv2627c, Rv2628, Rv3126c, Rv3129, Rv3130c, Rv3132c, Rv3133c (DosR), Rv0080, Rv1737c (NarX), Rv1735c and Rv1736c (NarK2), and analogues, homologues or fragments thereof.
1 Assignment
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Accused Products
Abstract
The invention identifies a narrow subset of Mycobacterium latency associated antigens and or epitopes that are capable of eliciting an immune response in vivo and in vitro in a mammal. The invention provides methods and compositions for detection and immunization against latent Mycobacterium infections. These compositions comprise those Mycobacterial latency antigens that are actually capable of eliciting an immune response in vivo, in mammals experiencing a latent Mycobacterium infection. More preferably the compositions comprise those antigens that are preferentially recognized by latently infected individuals and which antigens are not, or to a much lesser extent, recognized in individuals having an active Mycobacterium infection or in individuals having Mycobacterium induced symptoms or diseases, such as in patients infected with M. tuberculosis suffering from tuberculosis disease (TB).
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Citations
19 Claims
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1. A method for inducing an immune response against a Mycobacterium infection in a vertebrate, the method comprising the step of administering to the vertebrate a composition comprising a source of one or more polypeptides selected from the group consisting of M. tuberculosis dormancy (DosR) regulon sequences:
- Rv0079, Rv0569, Rv1733c, Rv1738, Rv1813c, Rv1996, Rv2007c, Rv2029c (PfkB), Rv2030c, Rv2031c (HspX), Rv2032, Rv2626c, Rv2627c, Rv2628, Rv3126c, Rv3129, Rv3130c, Rv3132c, Rv3133c (DosR), Rv0080, Rv1737c (NarX), Rv1735c and Rv1736c (NarK2), and analogues, homologues or fragments thereof.
- View Dependent Claims (2, 3, 4, 19)
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5. A composition for immunization against Mycobacterium infections comprising a source of one or more polypeptides selected from the group consisting of TB dormancy (DosR) regulon sequences:
- Rv0079, Rv0569, Rv1733c, Rv1738, Rv1813c, Rv1996, Rv2007c, Rv2029c (PfkB), Rv2030c, Rv2031c (HspX), Rv2032, Rv2626c, Rv2627c, Rv2628, Rv3126c, Rv3129, Rv3130c, Rv3132c and Rv3133c (DosR), Rv0080, Rv1737c (NarX), Rv1735c and Rv1736c (NarK2), and analogues, homologues or fragments thereof, and optionally comprising an adjuvant.
- View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method for diagnosing Mycobacterium infections in a subject comprising the steps of:
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a) contacting a sample of isolated bodily fluid and/or isolated white blood cells of the subject with one or more polypeptides or fragments thereof selected from the group of proteins encoded by the TB dormancy regulon consisting of;
Rv1733c, Rv2029c (PfkB), Rv2627c, Rv2628, Rv0080, Rv1737c (NarK2), Rv1735c and Rv1736c (NarX); and
,b) detecting a humoral immune response by binding of antibodies to said polypeptide(s) or fragments thereof being indicative of Mycobacterium infection and/or; c) detecting a cellular immune response by specific proliferation and/or cytokine production and/or expression of extra- or intracellular activation markers. - View Dependent Claims (18)
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Specification