METHOD FOR DEVELOPING, TESTING, AND USING ASSOCIATES OF MACROMOLECULES AND COMPLEX AGGREGATES FOR IMPROVED PAYLOAD AND CONTROLLABLE DE/ASSOCIATION RATES
First Claim
1. A pharmaceutical composition comprising:
- (i) a bilayer vesicle consisting essentially of;
at least one phosphatidylcholine; and
a nonionic detergent, at least one detergent;
(ii) at least one macromolecule; and
(iii) an aqueous liquid medium, wherein the macromolecule resides on the surface of the vesicle.
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Abstract
This invention describes the principles and procedures suitable for developing, testing, manufacturing, and using combinations of various amphipatic, if necessary modified, macromolecules (such as polypeptides, proteins, etc.) or other chain molecules (such as suitable, e.g. partly hydrophobised, polynucleotides or polysaccharides) with the aggregates which comprise a mixture of polar and/or charged amphipats and form extended surfaces that can be freely suspended or supported. The described methods can be utilised for the optimisation of aggregates that, after association with chain molecules exerting some activity or a useful function, are suitable for the application in vitro or in vivo, for example, in the fields of drug delivery, diagnostics or bio/catalysis. As special examples, mixtures of vesicular droplets consisting of lipids loaded (associated) with insulin, interferon, interleukin, nerve growth factor, calcitonin, and an immunoglobulin, etc., are described.
14 Citations
79 Claims
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1. A pharmaceutical composition comprising:
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(i) a bilayer vesicle consisting essentially of; at least one phosphatidylcholine; and a nonionic detergent, at least one detergent; (ii) at least one macromolecule; and (iii) an aqueous liquid medium, wherein the macromolecule resides on the surface of the vesicle. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 75, 76, 77, 78, 79)
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12. (canceled)
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24-28. -28. (canceled)
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44-74. -74. (canceled)
Specification