Production of Glucosinolates from Agricultural By-Products & Waste
First Claim
1. A method of extracting glucosinolates from glucosinolate-containing plant material, said method comprising:
- (i) providing a mixture of glucosinolate-containing plant material and liquid;
(ii) heating the mixture for a time and temperature sufficient to inactivate enzymes in the glucosinolate-containing plant material to form a heat inactivated mixture;
(iii) contacting the heat inactivated mixture with an anion exchange membrane for about 4 to about 24 hours and a temperature of about 2 to about 7°
C., whereby at least a portion of the glucosinolates are absorbed onto the anion exchange membrane; and
(iv) contacting the anion exchange membrane having absorbed glucosinolates with an aqueous solution for a time sufficient to release the extracted glucosinolates from the anion exchange membrane to form an aqueous media containing the glucosinolates.
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Abstract
The present invention relates to a process for producing glucosinolates, particularly glucoraphanin, from cruciferous plants. More particularly, a method is provided for producing glucosinolates from agricultural by-products and waste. The general method comprises providing a mixture of glucosinolate-containing plant material and liquid, heating the mixture to inactivate enzymes in the plant material, contacting the heat inactivated mixture with an anion exchange membrane whereby at least a portion of the glucosinolates are extracted from the liquid and absorbed onto the anion exchange membrane, and releasing the glucosinolates from the anion exchange membrane. Preferably, the extraction and release steps are repeated at least once. The glucosinolates produce by the method of the invention may be incorporated into a variety of food products, pharmaceuticals, and health supplements.
99 Citations
26 Claims
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1. A method of extracting glucosinolates from glucosinolate-containing plant material, said method comprising:
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(i) providing a mixture of glucosinolate-containing plant material and liquid; (ii) heating the mixture for a time and temperature sufficient to inactivate enzymes in the glucosinolate-containing plant material to form a heat inactivated mixture; (iii) contacting the heat inactivated mixture with an anion exchange membrane for about 4 to about 24 hours and a temperature of about 2 to about 7°
C., whereby at least a portion of the glucosinolates are absorbed onto the anion exchange membrane; and(iv) contacting the anion exchange membrane having absorbed glucosinolates with an aqueous solution for a time sufficient to release the extracted glucosinolates from the anion exchange membrane to form an aqueous media containing the glucosinolates. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of extracting glucosinolates from glucosinolate-containing plant material, said method comprising:
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(i) providing a mixture of glucosinolate-containing plant material and liquid; (ii) heating the mixture for a time and temperature sufficient to inactivate enzymes in the glucosinolate-containing plant material to form a heat inactivated mixture; (iii) contacting the heat inactivated mixture with an anion exchange membrane for about 4 to about 24 hours and a temperature of about 2 to about 7°
C., whereby at least a portion of the glucosinolates are absorbed onto the anion exchange membrane;(iv) contacting the anion exchange membrane having absorbed glucosinolates with an aqueous solution for a time sufficient to release the extracted glucosinolates from the anion exchange membrane to form an aqueous media containing the glucosinolates; (v) contacting the heat inactivated mixture with an anion exchange membrane for about 4 to about 24 hours at a temperature of about 2 to about 7°
C., whereby at least a portion of the glucosinolates are absorbed onto the anion exchange membrane;(vi) contacting the anion exchange membrane of step (v) having absorbed glucosinolates with an aqueous solution for a time sufficient to release the extracted glucosinolates from the anion exchange membrane to form an aqueous media containing the glucosinolates. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A glucosinolate extract formed by a process comprising:
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(i) providing a mixture of glucosinolate-containing plant material and liquid; (ii) heating the mixture for a time and temperature sufficient to inactivate enzymes in the glucosinolate-containing plant material to form a heat inactivated mixture; (iii) contacting the heat inactivated mixture with an anion exchange membrane for about 4 to about 24 hours and a temperature of about 2 to about 7°
C., whereby at least a portion of the glucosinolates are absorbed onto the anion exchange membrane; and(iv) contacting the anion exchange membrane having absorbed glucosinolates with an aqueous solution for a time sufficient to release the extracted glucosinolates from the anion exchange membrane to form an aqueous media containing the glucosinolates. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification