System and method for multiplex liquid handling

• US 20080311585A1
• Filed: 06/06/2008
• Published: 12/18/2008
• Est. Priority Date: 11/02/2005
• Status: Abandoned Application

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Gao, Chuan, Yamamoto, Melvin, Nguyen, Devin
• Application Number:
  US12/134,857
• Publication Number:
  US 20080311585A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01F 31/65 the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/81 Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
• CPC Class Codes: B01F 33/813 mixing simultaneously in two or more mixing receptacles
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 35/08 : using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

The present invention generally relates to microfabricated devices for carrying out and controlling chemical reactions and analysis. In particular, the present invention provides systems, methods, devices and computer software products related to multiplex liquid handling systems utilizing lab cards related to biological assays.

Claims

1. A microfabricated device for simultaneously transporting a plurality of aliquots of at least one fluid a known distance in a lab card the device comprising:

a first and a second structure separated from a common chamber by a flexible diaphragm, wherein the flexible diaphragm covers the first and second structures, wherein the first and second structures are each a chamber having a known volume and wherein the first and second structures are each in connection with a different reaction port; and

a valve that can be actuated to allow a pressurized gas to fill the common chamber to deform the flexible diaphragm into the first and second structures so that the aliquots of the fluid are transported the known distance in the lab card.

2. The microfabricated device of claim 1, wherein the flexible diaphragm is comprised of a silicone rubber.

3. The microfabricated device of claim 1, wherein the flexible diaphragm is bonded to the microfabricated device by an adhesive.

4. A microfabricated device for simultaneously mixing a plurality of aliquots of a mixture of fluids in a lab card, the device comprising:

a plurality of pairs of a first structure and a second structure wherein the first structures are separated from the second structures by at least one flexible diaphragm, wherein the first and second structures are each a chamber having a known volume, wherein the first structures are each in connection with a reaction port, wherein the second structures are connected by a common chamber;

a first valve that can be actuated to allow a pressurized gas to fill the common chamber and deform the flexible diaphragm into the first structures so that the aliquots of the mixture of fluids are transported a known distance from point A to point B in the lab card; and

a second valve that can be actuated to create a vacuum in the common chamber and deform the flexible diaphragm into the second structures so that the aliquots of the mixture of fluids are transported back a known distance from point B to point A, wherein the transporting from point A to point B, back to point A results in mixing of the mixture of fluids in the lab card.

5. The microfabricated device of claim 4, wherein the flexible diaphragm is comprised of a silicone rubber.

6. The microfabricated device of claim 4, wherein the flexible diaphragm is bonded to the microfabricated device by an adhesive.

7. A microfabricated device for simultaneously transporting a plurality of aliquots of a fluid a known distance in a lab card, the device comprising:

a) a plurality of pairs of a first and a second structure connected by one common channel, wherein the first structures are separated from a first common chamber by a first flexible diaphragm, wherein the first structures are each in connection with a reaction port, wherein the first structures are chambers having a known volume, and the second structures are separated from a second common chamber by a second flexible diaphragm, wherein the second structures are connected to a vent;

b) a first valve and a second valve, wherein the second valve can be actuated to allow a pressurized gas to fill the second common chamber while the first valve is deactuated to close the vent and a plurality of openings between the common channels and the second structures, wherein the second valve is connected to the second common chamber and the first valve is connected to the first common chamber, wherein the first valve can be actuated to allow the pressurized gas to fill the first common chamber while maintaining the actuating step of the second valve to initiate transporting of the aliquots of the fluid a know distance in the lab card, wherein the first valve can be deactuated while maintaining the actuating step of the second valve to finish transporting the aliquots of the fluid a known distance in the lab card, and wherein the second valve can be deactuated while maintaining the deactuating step of the first valve to equilibrate the pressure in the lab card so that the aliquots of the fluid are transported in the lab card.

8. The microfabricated device of claim 7, wherein the flexible diaphragm is comprised of a silicone rubber.

9. The microfabricated device of claim 7, wherein the flexible diaphragm is bondd to the microfabricated device by an adhesive.

10. A method of simultaneously transporting a plurality of aliquots of a fluid a known distance in a lab card, the method comprising:

providing a microfabricated device having at least a first and a second structure separated from a common chamber by a flexible diaphragm, wherein the flexible diaphragm covers the first and second structures, wherein the first and second structures are a plurality of chambers having a known volume and wherein the first and second structures are in connection with a different reaction port; and

actuating a valve to allow a pressurized gas to fill the common chamber to deform the flexible diaphragm into the first and second structures to transport the aliquots of the fluid the known distance in the lab card.

11. The method of claim 10, wherein the flexible diaphragm is comprised of a silicone rubber.

12. The method of claim 10, wherein the flexible diaphragm is bonded to the microfabricated device by an adhesive.

13. A method of simultaneously mixing a plurality of aliquots of a mixture of fluids in a miniature lab card, the method comprising:

a) providing a microfabricated device having a plurality of pairs of a first structure and a second structure wherein the first structures are separated from the second structures by at least one flexible diaphragm, wherein the first and second structures are each a chamber having a known volume, wherein the first structures are each in connection with a reaction port, wherein the second structures are connected by a common chamber;

b) actuating a first valve to allow a pressurized gas to fill the common chamber to deform the flexible diaphragm into the first structures so that the aliquots of the mixture of fluids are transported a known distance from point A to point B in the lab card; and

c) actuating a second valve to create a vacuum in the common chamber to deform the flexible diaphragm into the second structures so that the aliquots of the mixture of fluids are transported back a known distance from point B to point A; and

d) repeating steps b and c so that the aliquots of the mixture of fluids are repeatedly transported from point A to point B, back to point A resulting in mixing of the mixture of fluids in the lab card.

14. The method of claim 13, wherein the flexible diaphragm is comprised of a silicone rubber.

15. The method of claim 13, wherein the flexible diaphragm is bonded to the microfabricated device by an adhesive.

16. A method of simultaneously transporting a plurality of aliquots of a fluid a known distance in a lab card, the method comprising:

a) providing a microfabricated device having a plurality of pairs of a first and a second structure connected by one common channel, wherein an opening is provided between the common channel and the second structure, wherein the second structures are connected to a vent;

wherein the second structures are separated from a second common chamber by a second flexible diaphragm, and wherein the first structures are separated from a first common chamber by a first flexible diaphragm, wherein the first structures are each in connection with a reaction port, wherein the first structures are chambers having a known volume;

b) actuating a second valve while a first valve is deactuated to allow a pressurized gas to fill the second common chamber wherein the flexible diaphragm is deformed into the second structure to close the vent and a plurality of openings between the common channels and the second structures by, wherein the second valve is connected to the second common chamber and the first valve is connected to the first common chamber;

c) actuating the first valve while maintaining the actuating step of the second valve to allow a pressurized gas to fill the first common chamber wherein the flexible diaphragm is deformed into the first structure to initiate transporting of the aliquots of the fluid a know distance in the lab card;

d) deactuating the first valve while maintaining the actuating step of the second valve to finish transporting the aliquots of the fluid a known distance in the lab card;

e) deactuating the second valve while maintaining the deactuating step of the first valve to equilibrate the pressure in the lab card; and

f) repeating the steps (b)-(e) to transport the aliquots of the fluid in the lab card.

17. The method of claim 16, wherein the flexible diaphragm is comprised of a silicone rubber.

18. The method of claim 16, wherein the flexible diaphragm is bonded to the microfabricated device by an adhesive.