ANTIBODIES AGAINST 25-HYDROXYVITAMIN D

• US 20080317764A1
• Filed: 03/21/2008
• Published: 12/25/2008
• Est. Priority Date: 09/29/2005
• Status: Abandoned Application

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Vogel, Rudolf, Horn, Nicole, Kyriatsoulis, Apostolos, Huber, Eramus, Kraus, Werner, Becker, Juergen
• Application Number:
  US12/053,172
• Publication Number:
  US 20080317764A1
• Time in Patent Office: Days
• US Class Current: 424/175.1
• CPC Class Codes: C07K 16/26 against hormones ; against hormone releasing or inhibiting factors
• CPC Class Codes: C07K 16/44 against material not provided for elsewhere , e.g. haptens, metals, DNA, RNA, amino acids

Abstract

The present invention concerns processes for producing antibodies against 25-hydroxyvitamin D, the antibodies produced according to the invention as well as methods for the detection of 25-hydroxyvitamin D using these antibodies.

Claims

1. A process for producing an antibody against 25-hydroxyvitamin D comprising the steps of

immunizing an animal with a conjugate comprising 25-hydroxyvitamin D₃ coupled to a carrier,

isolating serum or plasma from the animal containing the antibody against 25-hydroxyvitamin D, and

purifying the antibody against 25-hydroxyvitamin D by immunosorption of the antibody to a matrix comprising 25-hydroxyvitamin D₂.

3. The process of claim 1 wherein the 25-hydroxyvitamin D₃ is coupled to the carrier at position 3 of the 25-hydroxyvitamin D₃.

5. The process of claim 1 wherein the 25-hydroxyvitamin D₂ is linked to the matrix via position 3 of the 25-hydroxyvitamin D₂.

2. A process for producing an antibody against 25-hydroxyvitamin D comprising the steps of

immunizing an animal with a conjugate comprising 25-hydroxyvitamin D₂ coupled to a carrier,

isolating serum or plasma from the animal containing the antibody against 25-hydroxyvitamin D, and

purifying the antibody against 25-hydroxyvitamin D contained in the serum or plasma by immunosorption of the antibody to a matrix comprising 25-hydroxyvitamin D₃.

4. The process of claim 2 wherein the 25-hydroxyvitamin D₂ is coupled to the carrier at position 3 of the 25-hydroxyvitamin D₂.

6. The process of claim 2 wherein the 25-hydroxyvitamin D₃ is linked to the matrix via position 3 of the 25-hydroxyvitamin D₃.

7. An antibody against 25-hydroxyvitamin D₃ which has a cross-reaction of 10% to 1000% with 25-hydroxyvitamin D₂.

9. A method for detection of 25-hydroxyvitamin D in a sample comprising the steps of

adding to said sample a detection antibody and an antigen whereby the antigen is a defined amount of 25-hydroxyvitamin D that competes with the 25-hydroxyvitamin D in the sample to bind with the detection antibody, whereby the detection antibody comprises the antibody of claim 7 and a moiety which produces a signal upon binding of the antibody to the antigen, and

determining the signal produced as a measure of the 25-hydroxyvitamin D in the sample.

11. A test kit for detection of 25-hydroxyvitamin D comprising the antibody of claim 7.

8. An antibody against 25-hydroxyvitamin D₃ which has a cross-reaction of 20% to 500% with 25-hydroxyvitamin D₂.

10. A method for detection of 25-hydroxyvitamin D in a sample comprising the steps of

adding to said sample a detection antibody and an antigen whereby the antigen is a defined amount of 25-hydroxyvitamin D that competes with the 25-hydroxyvitamin D in the sample to bind with the detection antibody, whereby the detection antibody comprises the antibody of claim 8 and a moiety which produces a signal upon binding of the antibody to the antigen and

determining the signal produced as a measure of the 25-hydroxyvitamin D in the sample.

12. A test kit for detection of 25-hydroxyvitamin D comprising the antibody of claim 8.