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Source tagging and normalization of DNA for parallel DNA sequencing, and direct measurement of mutation rates using the same

  • US 20080318233A1
  • Filed: 03/31/2008
  • Published: 12/25/2008
  • Est. Priority Date: 03/30/2007
  • Status: Abandoned Application
First Claim
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1. A method to tag one or more DNA fragments from one or more subjects to investigate one or more loci of interest comprising:

  • (a) normalizing the concentration of the one or more DNA fragments;

    (b) pooling the one or more DNA fragments;

    (c) ligating distinct identification linker tags to each of the DNA fragments;

    (d) optionally pooling the distinctly tagged DNA fragments;

    (e) processing the distinctly tagged DNA fragments through parallel sequencing; and

    (f) using the identification linker tag to differentiate the one or more DNA fragments to investigate one or more loci of interest.

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