APPARATUS AND METHOD FOR PURIFICATION OF NUCLEIC ACIDS BY PHASE SEPARATION USING LASER AND BEADS
First Claim
1. A nucleic acid purification apparatus of cells or viruses comprising:
- a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced;
a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary;
a laser generator attached to the capillary and supplying a laser to the capillary; and
a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall.
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Abstract
An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.
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Citations
20 Claims
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1. A nucleic acid purification apparatus of cells or viruses comprising:
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a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. - View Dependent Claims (2, 3, 4)
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5. A method of continuously performing purification and amplification of nucleic acids, the method comprising:
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injecting a solution containing cells or viruses to a capillary-shaped container containing magnetic beads; vibrating the magnetic beads; irradiating a laser beam onto the magnetic beads to disrupt the cells or viruses and binding compounds in the resulting cell or virus lysate to the magnetic beads; fixing the magnetic beads, to which the compounds in the cell or virus lysate are bound, to a capillary-shaped container wall by means of a magnetic force generator; and obtaining a solution without the magnetic bead and transferring the resulting solution to a amplification chamber through a channel connecting the container and the amplification chamber to perform amplification. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification