METHODS AND COMPOSITIONS FOR TAGGING AND IDENTIFYING POLYNUCLEOTIDES
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Abstract
The invention provides methods and compositions for attaching oligonucleotide tags to polynucleotides for the purpose of carrying out analytical assays in parallel and for decoding the oligonucleotide tags of polynucleotides selected in such assays. Words, or subunits, of oligonucleotide tags index submixtues in successively more complex sets of submixtures (referred to herein as “tiers” of submixtures) that a polynucleotide goes through while successive words are added to a growing tag. By identifying each word of an oligonucleotide tag, a series of submixtures is identified including the first submixture that contains only a single polynucleotide, thereby providing the identity of the selected polynucleotide. The analysis of the words of an oligonucleotide tag can be carried out in parallel, e.g. by specific hybridization of the oligonucleotide tag to its tag complement on an addressable array; or such analysis can be carried out serially by successive specific hybridizations of labeled word complements, or the like.
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Citations
41 Claims
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1-21. -21. (canceled)
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22. A method of identifying a polynucleotide selected from a mixture of tagged polynucleotides, the method comprising the steps of:
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selecting one or more tagged polynucleotide from a mixture of tagged polynucleotides, said mixture comprising polynucleotides from a plurality of different polynucleotide samples wherein the polynucleotides in each of said different polynucleotide samples are tagged with a unique oligonucleotide tag comprising one or more words; and determining the nucleotide sequence of at least one word of the unique oligonucleotide tag on said selected one or more tagged polynucleotide to identify from which of said plurality of different polynucleotide samples each of said selected one or more tagged polynucleotide is derived. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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Specification