Implantable Power Sources and Sensors
First Claim
3. Apparatus for detecting a concentration of a substance in a subject, the apparatus comprising a housing adapted to be implanted in the subject, the housing comprising:
- a fluorescence resonance energy transfer (FRET) measurement device; and
cells genetically engineered to produce, in situ, a FRET protein having a FRET complex comprising a fluorescent protein donor, a fluorescent protein acceptor, and a binding site for the substance.
1 Assignment
0 Petitions
Accused Products
Abstract
A protein is provided, including a glucose binding site, cyan fluorescent protein (CFP), and yellow fluorescent protein (YFP). The protein is configured such that binding of glucose to the glucose binding site causes a reduction in a distance between the CFP and the YFP. Substance monitoring apparatus (210) is also provided, including a semi-permeable barrier (212), adapted to be implanted in a body of a subject and to allow passage therethrough of a substance, while inhibiting passage therethrough of immune cells; and microorganisms (214), disposed within the semi-permeable barrier (212) so as to produce a measurable response to a level of the substance. A sensor (220) is adapted to measure the measurable response and not to measure a response of any mammalian cells that may be disposed within the semi-permeable barrier (212).
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Citations
57 Claims
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3. Apparatus for detecting a concentration of a substance in a subject, the apparatus comprising a housing adapted to be implanted in the subject, the housing comprising:
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a fluorescence resonance energy transfer (FRET) measurement device; and cells genetically engineered to produce, in situ, a FRET protein having a FRET complex comprising a fluorescent protein donor, a fluorescent protein acceptor, and a binding site for the substance. - View Dependent Claims (1, 2, 18, 20, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57)
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4-17. -17. (canceled)
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19. (canceled)
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21-41. -41. (canceled)
Specification