COMPOSITIONS AND METHODS FOR GENETIC MODIFICATION OF PLANTS

• US 20080320617A1
• Filed: 08/01/2008
• Published: 12/25/2008
• Est. Priority Date: 11/18/1997
• Status: Active Grant

• Current Assignee: Pioneer Hi Bred International Inc (Corteva, Inc.)
• Original Assignee: Pioneer Hi Bred International Inc (Corteva, Inc.)
• Inventors: Baszczynski, Christopher L., Bowen, Benjamin A., Tagliani, Laura A., Peterson, David J.
• Granted Patent: US 8,536,420 B2
• Time in Patent Office: Days
• US Class Current: 800/320.1
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 15/8203 Virus mediated transformation
• CPC Class Codes: C12N 15/8205 Agrobacterium mediated transformation
• CPC Class Codes: C12N 15/8213 Targeted insertion of genes into the plant genome by homologous recombination
• CPC Class Codes: C12N 15/90 Stable introduction of foreign DNA into chromosome
• CPC Class Codes: C12N 9/00 Enzymes; Proenzymes; Compositions thereof preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113; Processes for preparing, activating, inhibiting, separating or purifying enzymes preparation of malt C12C1/00

Abstract

Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.

Claims

1. A maize plant cell having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by a first and a second non-identical recombination site, wherein said first and said second non-identical recombination site can recombine in said maize plant cell with their identical recombination site in the presence of the same recombinase.

2. The maize plant cell of claim 1, wherein said non-identical recombination sites are selected from the group consisting of an FRT site, a mutant FRT site, a LOX site, and a mutant LOX site.

3. The maize plant of claim 1, wherein the genome further comprises an expression cassette comprising a nucleotide sequence encoding a recombinase.

4. The maize plant cell of claim 3, wherein said recombinase comprises FLP recombinase.

5. The maize plant cell of claim 4, wherein said FLP recombinase comprises maize preferred codons.

6. The maize plant cell of claim 3, wherein said recombinase comprises Cre recombinase.

7. The maize plant cell of claim 6, wherein said Cre recombinase comprises maize preferred codons.

8. A maize plant having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by a first and a second non-identical recombination site, wherein said first and said second non-identical recombination site can recombine in said maize plant with their identical recombination site in the presence of the same recombinase.

9. The maize plant of claim 8, wherein said non-identical recombination sites are selected from the group consisting of an FRT site, a mutant FRT site, a LOX site, and a mutant LOX site.

10. The maize plant of claim 9, wherein the genome further comprises an expression cassette comprising a nucleotide sequence encoding a recombinase.

11. The maize plant of claim 10, wherein said recombinase comprises FLP recombinase.

12. The maize plant cell of claim 11, wherein said FLP recombinase comprises maize preferred codons.

13. The maize plant of claim 10, wherein said recombinase comprises Cre recombinase.

14. The maize plant of claim 13, wherein said Cre recombinase comprises maize preferred codons.

15. The transformed seed of the plant of claim 8.