Small Volume in Vitro Analyte Sensor
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Accused Products
Abstract
A sensor designed to determine the amount and concentration of analyte in a sample having a volume of less than about 1 μL. The sensor has a working electrode coated with a non-leachable redox mediator. The redox mediator acts as an electron transfer agent between the analyte and the electrode. In addition, a second electron transfer agent, such as an enzyme, can be added to facilitate the electrooxidation or electroreduction of the analyte. The redox mediator is typically a redox compound bound to a polymer. The preferred redox mediators are air-oxidizable.
The amount of analyte can be determined by coulometry, amperometry, voltammetry, and potentiometry. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is provided as a second electron transfer agent.
101 Citations
160 Claims
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1-132. -132. (canceled)
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133. A method for determining a concentration of an analyte in a body fluid of a patient, comprising the steps of:
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creating an unassisted flow of a body fluid from the patient; transporting a portion of the body fluid into an analyte sensor configured and arranged to determine the concentration of the analyte from 1000 nL or less of body fluid; holding the sample within a sample chamber of the analyte sensor; and determining the concentration of the analyte in the body fluid from the portion of the body fluid transported into the analyte sensor. - View Dependent Claims (134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160)
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Specification