METHOD AND DEVICE FOR IDENTIFYING AN UNKNOWN SUBSTANCE

• US 20090002699A1
• Filed: 06/28/2007
• Published: 01/01/2009
• Est. Priority Date: 06/28/2007
• Status: Abandoned Application

• Current Assignee: Morpho Detection (Safran SA)
• Original Assignee: Morpho Detection (Safran SA)
• Inventors: Salinas, Ruben Fernando, Lee, Yuan-Hsiang, Engel, Patrick Ronald, Sutherland, William Scott
• Application Number:
  US11/769,920
• Publication Number:
  US 20090002699A1
• Time in Patent Office: Days
• US Class Current: 356/301
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0264 Electrical interface; User interface
• CPC Class Codes: G01J 3/0272 Handheld
• CPC Class Codes: G01J 3/0291 Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 2333/32 from Bacillus (G)
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/588 with semiconductor nanocrystal label, e.g. quantum dots

Abstract

A device for identifying an unknown substance includes an optical source configured to direct a laser excitation beam at the unknown substance. A detector is configured to detect scattered light from the unknown substance and generate at least one signal representative of a scattering spectrum corresponding to at least one chemical within the unknown substance. A microprocessor is in signal communication with the detector and configured to generate a pattern representative of the at least one chemical in response to the at least one signal received from the detector to identify the chemical composition of the unknown substance. The device is configured to complete a bioassay to identify a biological nature of the unknown substance.

Claims

1. A device for identifying an unknown substance, said device comprising:

an optical source configured to direct a laser excitation beam at the unknown substance;

a detector configured to detect scattered light from the unknown substance and generate at least one signal representative of a scattering spectrum corresponding to at least one chemical within the unknown substance; and

a microprocessor in signal communication with said detector, said microprocessor configured to generate a pattern representative of the at least one chemical in response to said at least one signal received from said detector to identify the chemical composition of the unknown substance, said device configured to complete a bioassay to identify a biological nature of the unknown substance.

2. A device in accordance with claim 1 wherein said optical source further comprises a probe configured to be in contact proximity with the unknown substance.

3. A device in accordance with claim 1 wherein said device utilizes Raman spectroscopy to identify the at least one chemical within the unknown substance.

4. A device in accordance with claim 1 further comprising an integrated data storage memory in communication with said microprocessor, said data storage memory comprising a wavelength and amplitude reference module for facilitating comparing said pattern with a plurality of patterns for known chemicals stored within said wavelength and amplitude reference module.

5. A device in accordance with claim 1 further comprising a bioassay assembly operatively coupled to said microprocessor, said bioassay assembly configured to complete the bioassay to facilitate identifying the biological nature of the unknown substance.

6. A device in accordance with claim 5 wherein said bioassay assembly further comprises:

an assay cartridge positionable in communication with said optical source, said assay cartridge defining a compartment configured to receive a sample portion of the unknown substance;

at least one reagent introduced into said compartment, said at least one reagent configured to selectively interact with a target biological substance within the sample portion to form a complex; and

a reader in signal communication with said optical source, said reader configured to detect scattered light from the complex and generate at least one signal representative of a scattering spectrum corresponding to the complex.

7. A device in accordance with claim 6 wherein said at least one reagent further comprises at least one nanotag including at least one of a chemically and biologically selective material against the target biological substance and at least one magnetic separation bead including the antibody.

8. A device in accordance with claim 7 wherein said device further comprises a magnetic source configured to generate a magnetic field with respect to said assay cartridge to facilitate collecting in a localized region within said compartment the complex comprising the target biological substance attached to said at least one nanotag and said at least one magnetic separation bead.

9. A device in accordance with claim 6 wherein said at least one reagent further comprises a plurality of SERS nanotags, each of said plurality of SERS nanotags comprising a particle configured to generate an enhanced Raman spectrum and a tag coupled to said particle.

10. A device in accordance with claim 9 wherein each said SERS nanotag is specific to one target biological substance, said microprocessor configured to process a scattering spectrum produced by each said SERS nanotag and identify the biological substance.

11. A device for identifying an unknown substance, said device comprising:

an optical source configured to direct a laser excitation beam at the unknown substance;

a detector configured to detect scattered light from the unknown substance and generate at least one signal representative of at least one Raman spectrum corresponding to a chemical within the unknown substance;

a microprocessor in signal communication with said detector, said microprocessor configured to process the at least one signal to facilitate identifying the chemical corresponding to the at least one Raman spectrum; and

a bioassay assembly operatively coupled to said microprocessor, said bioassay assembly configured to facilitate completing a bioassay to identify a biological nature of the unknown substance.

12. A device in accordance with claim 11 wherein said bioassay assembly further comprises:

an assay cartridge positionable in communication with said optical source, said assay cartridge defining a compartment containing a liquid and configured to receive a sample portion of the unknown substance such that the sample portion is dispersed within the liquid;

at least one reagent introduced into the liquid, said at least one reagent configured to selectively interact with a target biological substance within the sample portion to form a complex; and

a reader in signal communication with said optical source, said reader configured to detect scattered light from the complex and generate at least one signal representative of a Raman spectrum corresponding to the complex.

13. A device in accordance with claim 12 wherein said at least one reagent further comprises a plurality of magnetic separation beads including an antibody against the target biological substance, said plurality of magnetic separation beads reactive to a magnetic field generated with respect to said assay cartridge to facilitate forming the complex comprising the target biological substance attached to said plurality of magnetic separation beads.

14. A device in accordance with claim 13 wherein said at least one reagent further comprises a plurality of SERS nanotags, each of said plurality of SERS nanotags comprising:

a gold core;

at least one Raman reporter molecule configured to generate a SERS signal;

a glass shell surrounding said gold core and said at least one Raman reporter molecule; and

an antibody label against the target biological substance coupled to an outer surface of said glass shell, each said SERS nanotag configured to attach to a corresponding target biological substance.

15. A device in accordance with claim 14 further comprising a microprocessor in signal communication with said reader, said microprocessor configured to identify a Raman spectrum produced by each said SERS nanotag corresponding to the target biological substance.

16. A device in accordance with claim 11 wherein said bioassay assembly further comprises a substrate defining a flow path, said substrate comprising a detection zone including at least one immobilized target-binding moiety directed against a target biological substance and a control zone disposed along the flow path down flow from said detection zone, said control zone including at least one immobilized particle-binding-moiety directed against a Raman-active nanotag.

17. A method for identifying an unknown substance using an integrated device, said method comprising:

directing a laser excitation beam from an optical source at the unknown substance;

detecting scattered light from the unknown substance;

generating at least one signal representative of a scattering spectrum corresponding to at least one chemical within the unknown substance;

processing the at least one signal with a microprocessor housed within the device to generate a pattern representative of the at least one chemical;

identifying the at least one chemical within the unknown substance; and

completing a bioassay to facilitate identifying a biological nature of the unknown substance.

18. A method in accordance with claim 17 identifying the at least one chemical within the unknown substance further comprises comparing the pattern with a plurality of patterns for known chemicals stored within a wavelength and amplitude module of the microprocessor.

19. A method in accordance with claim 17 wherein completing a bioassay further comprises:

dispersing a sample portion of the unknown substance within an assay cartridge compartment containing a liquid to form a solution;

introducing at least one reagent into the solution, the at least one reagent configured to selectively interact with a target biological substance;

separating a complex including the target biological substance attached to the at least one reagent from the solution; and

scanning the complex with the optical source.

20. A method in accordance with claim 19 wherein introducing at least one reagent into the assay cartridge compartment further comprises introducing into the solution at least one SERS nanotag including an antibody against the target biological substance and at least one magnetic separation bead including the antibody against the target biological substance, and separating a complex including the target biological substance attached to the at least one reagent from the solution further comprises collecting in a localized region within the assay cartridge compartment the complex comprising the target biological substance attached to the at least one SERS nanotag and the at least one magnetic separation bead.

21. A method in accordance with claim 20 further comprising:

detecting scattered light from the complex;

generating at least one signal representative of a Raman spectrum corresponding to the at least one SERS nanotag; and

comparing the Raman spectrum with a plurality of Raman spectra for known biological substances stored within the microprocessor to identify the biological substance.