METHODS AND SYSTEMS FOR QUANTIFYING ISOBARIC LABELS AND PEPTIDES
First Claim
Patent Images
1. A method of quantifying two or more isobaric labels comprising:
- applying a mathematical function to at least two isobaric label fragment peaks in a mass spectrum to provide a fitted curve; and
determining a relative amount of each isobaric label from the fitted curve.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods and systems for quantifying at least two isobaric labels and/or peptides labeled with different isobaric labels are disclosed. In certain examples, the method comprises fitting a mathematical function to at least two isobaric label fragment peaks in a mass spectrum to quantify simultaneously an amount of at least one peptide present in two different samples.
-
Citations
23 Claims
-
1. A method of quantifying two or more isobaric labels comprising:
-
applying a mathematical function to at least two isobaric label fragment peaks in a mass spectrum to provide a fitted curve; and determining a relative amount of each isobaric label from the fitted curve. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A method of quantifying peptides comprising:
-
subjecting a sample to fragmentation in a mass spectrometer, the sample comprising a first peptide labeled with a first isobaric label and a second peptide labeled with a second isobaric label, in which fragmentation in the mass spectrometer provides a low mass fragment peak and a high mass fragment peak for each of the first and second isobaric labels; applying a mathematical function to at least one of the low mass fragment peaks or the high mass fragment peaks to provide a fitted curve; and determining a relative amount of each of the first peptide and the second peptide from the fitted curve. - View Dependent Claims (10, 11, 12, 13, 14)
-
-
15. A mass spectrometer comprising:
-
a first stage configured to select at least two peptides labeled with two, different isobaric labels; a second stage in fluid communication with the first stage, the second stage configured to fragment the two, different isobaric labels of the selected at least two peptides to provide two low mass fragments and two high mass fragments; a processing device electrically coupled to the second stage, the processing device configured to quantify simultaneously the two or more peptides. - View Dependent Claims (16, 17, 18)
-
-
19. A kit comprising:
-
an isobaric label; a computer readable medium comprising an algorithm configured to quantify simultaneously at least two peptides; and instructions for using the isobaric label and the algorithm to quantify peptides in a sample. - View Dependent Claims (20, 21)
-
-
22. A method of quantifying at least two peptides labeled with different isobaric labels, the method comprising fitting a mathematical function to two isobaric label fragment peaks in a mass spectrum to quantify simultaneously a relative amount of the at least two peptides present in a sample.
-
23. A computer readable medium comprising an algorithm for simultaneously quantifying at least two peptides, the algorithm configured to fit a mathematical function to at least two isobaric label fragment peaks in a mass spectrum to quantify simultaneously a relative amount of the at least two peptides.
Specification