Measurement and uses of oxidative stress

• US 20090004686A1
• Filed: 05/16/2008
• Published: 01/01/2009
• Est. Priority Date: 05/18/2007
• Status: Active Grant

• Current Assignee: Caerus Biotechnologies
• Original Assignee: Institute For Molecular Medicine The (INSTITUTE FOR MOLECULAR MEDICINE)
• Inventors: Bar-Or, David, Bar-Or, Raphael
• Granted Patent: US 9,063,070 B2
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 33/50 Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q

Abstract

The present invention provides a method of determining the overall oxidative status of a body fluid or a tissue of a patient by measuring the oxidation-reduction potential (ORP) of the body fluid or tissue. The method has been found to be useful in the diagnosis, evaluation and monitoring of patients who have suffered a trauma (such as a head injury), patients suspected of being critically-ill, patients who have a viral infection, and patients suspected of having a myocardial infarction. The method has also been found useful in monitoring and evaluating exercise performance in patients. In addition, the method has been found useful in monitoring and evaluating stored blood products and patients who will receive such a product.

Claims

1. A method of diagnosing, evaluating or monitoring a patient who has suffered a trauma comprising the following steps:

a. measuring the oxidation-reduction potential (ORP) of a body fluid of the patient, a tissue of the patient or both; and

b. performing one or both of the following steps;

i. determining if the ORP is significantly different than the ORP of the same body fluid, tissue or both from normal patients; and

/or

ii. if the ORP of the body fluid, tissue or both of the patient has been measured at least one time prior to the current measurement, determining if the ORP has increased or decreased compared to the prior measurement(s).

2. The method of claim 1 wherein the ORP is monitored to determine the efficacy of a treatment of the patient.

3. The method of claim 1 wherein the ORP is monitored to determine whether the condition of the patient is improving or worsening.

4. The method of any one of claims 1-3 wherein the ORP is measured once per day or a plurality of times per day.

5. The method of any one of claims 1-3 wherein the ORP is measured in a body fluid, and the body fluid is plasma.

6. The method of any one of claims 1-3 wherein the trauma is a head injury.

7. The method of claim 6 wherein the ORP is measured once per day or a plurality of times per day.

8. The method of claim 6 wherein the ORP is measured in a body fluid, and the body fluid is plasma.

9. The method of claim 6 wherein the ORP is measured in a body fluid, and the body fluid is cerebrospinal fluid.

10. A method of diagnosing, evaluating or monitoring a patient who has a viral infection comprising the following steps:

a. measuring the oxidation-reduction potential (ORP) of a body fluid of the patient, a tissue of the patient or both; and

b. performing one or both of the following steps;

i. determining if the ORP is significantly different than the ORP of the same body fluid, tissue or both from normal patients; and

/or

ii. if the ORP of the body fluid, tissue or both of the patient has been measured at least one time prior to the current measurement, determining if the ORP has increased or decreased compared to the prior measurement(s).

11. The method of claim 10 wherein the ORP is monitored to determine the efficacy of a treatment of the patient.

12. The method of claim 10 wherein the ORP is monitored to determine whether the condition of the patient is improving or worsening.

13. The method of any one of claims 10-12 wherein the ORP is measured once per day or a plurality of times per day.

14. The method of any one of claims 10-12 wherein the ORP is measured in a body fluid, and the body fluid is plasma.

15. A method of diagnosing, evaluating or monitoring a patient suspected of being critically-ill or of evaluating or monitoring a patient who has been diagnosed as being critically ill, the method comprising the following steps:

a. measuring the oxidation-reduction potential (ORP) of a body fluid of the patient, a tissue of the patient or both; and

b. performing one or both of the following steps;

i. determining if the ORP is significantly different than the ORP of the same body fluid, tissue or both from normal patients; and

/or

ii. if the ORP of the body fluid, tissue or both of the patient has been measured at least one time prior to the current measurement, determining if the ORP has increased or decreased compared to the prior measurement(s).

16. The method of claim 15 wherein the ORP is used as part of an evaluation to determine whether the patient is or is not critically ill.

17. The method of claim 15 wherein the ORP is monitored to determine the efficacy of a treatment of the patient.

18. The method of claim 15 wherein the ORP is monitored to determine whether the condition of the patient is improving or worsening.

19. The method of any one of claim 15-18 wherein the ORP is measured once per day or a plurality of times per day.

20. The method of any one of claims 15-18 wherein the ORP is measured in a body fluid, and the body fluid is plasma.

21. A method of diagnosing, evaluating or monitoring a patient suspected of having a myocardial infarction or of evaluating or monitoring a patient who has been diagnosed as having a myocardial infarction, the method comprising the following steps:

a. measuring the oxidation-reduction potential (ORP) of a body fluid of the patient, a tissue of the patient or both; and

b. performing one or both of the following steps;

i. determining if the ORP is significantly different than the ORP of the same body fluid, tissue or both from normal patients; and

/or

ii. if the ORP of the body fluid, tissue or both of the patient has been measured at least one time prior to the current measurement, determining if the ORP has increased or decreased compared to the prior measurement(s).

22. The method of claim 21 wherein the ORP is used as part of an evaluation to determine whether the patient is or is not having a myocardial infarction.

23. The method of claim 21 wherein the ORP is monitored to determine the efficacy of a treatment of the patient.

24. The method of claim 22 wherein the ORP is measured once per day or a plurality of times per day.

25. The method of any one of claims 21-24 wherein the ORP is measured in a body fluid, and the body fluid is plasma.

26. A method of evaluating the exercise performance of a patient comprising:

a. measuring the oxidation-reduction potential (ORP) of a body fluid of the patient, a tissue of the patient or both before and after exercise and determining if the ORP is significantly different after exercise as compared to before exercise;

or

b. measuring the ORP of a body fluid of the patient, a tissue of the patient or both before and during exercise and determining if the ORP is significantly different during exercise as compared to before exercise;

or

c. measuring the ORP of a body fluid of the patient, a tissue of the patient or both before, during and after exercise and determining if the ORP is significantly different during exercise, after exercise or both, as compared to before exercise.

28. A method of evaluating or monitoring a blood product which is stored comprising:

a. measuring the oxidation-reduction potential (ORP) of the blood product after it has been stored for one or more days; and

b. determining if the ORP of the stored blood product is significantly different than the ORP of the same blood product immediately after it was obtained from a donor.

32. The method of any one of claims 28-31 further comprising:

c. measuring the oxidation-reduction potential (ORP) of a body fluid of a patient who is to receive the blood product, a tissue of the patient or both; and

d. determining if the ORP is significantly different than the ORP level in the same body fluid, tissue or both from normal patients.

31. The method of claim 31 wherein the ORP of the blood product is measured once per day during storage.

27. The method of claim 29 wherein the ORP is measured in a body fluid, and the body fluid is plasma.

29. The method of claim 31 wherein the blood product is blood.

30. The method of claim 31 wherein the blood product is packed red blood cells.