MULTIPLEX DATA COLLECTION AND ANALYSIS IN BIOANALYTE DETECTION

• US 20090005260A1
• Filed: 06/26/2008
• Published: 01/01/2009
• Est. Priority Date: 09/01/2005
• Status: Active Grant

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Yamakawa, Mineo, Su, Xing, Ma, Qing, Jones, Richard, Sun, Lei, Zhang, Jingwu, Koo, Tae-Woong
• Granted Patent: US 8,062,841 B2
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a single spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

Claims

1-45. -45. (canceled)

46. A method comprising contacting a sample comprising an analyte with a reagent comprising first and second binding partners and first and second reporters, and forming a complex comprising the first binding partner, the first reporter associated with the first binding partner, the analyte, the second binding partner, and the second reporter associated with the second binding partner.

47. The method of claim 46, wherein the complex is formed in solution or on a surface.

48. The method of claim 46, further comprising placing a portion of the complex to a detection site.

49. The method of claim 48, wherein the detection site is a cavity in a MFC or a spot of a microarray.

50. The method of claim 48, further comprising collecting an electromagnetic spectrum from the portion of the complex in the detection site.

51. The method of claim 50, wherein the electromagnetic spectrum is a Raman spectrum.

52. The method of claim 50, further comprising de-convoluting the electromagnetic spectrum by a computer implemented system to identify the first and second reporters and providing analyte information based on the identity of the first and second reporters.

53. The method of claim 46, wherein the analyte is a bioanalyte containing information to detect a disease.

54. The method of claim 46, wherein the first reporter or the second reporter comprises a quantum dot, a Raman tag, a fluorescent tag, a COIN, or a magnetic COIN.

55. The method of claim 54, wherein the magnetic COIN comprises a metal particle with at least one Raman active organic compound adsorbed on the metal particle.

56. A method comprising contacting a sample comprising an analyte with a first binding partner immobilized on a surface and contacting a reagent comprising second binding partner and a reporter, and forming a complex comprising the first binding partner, the analyte, the second binding partner, reporter associated with the second reporter associated with the second binding partner.

57. The method of claim 56, wherein the surface is that of a spot enclosed in a MFC.

58. The method of claim 56, further comprising collecting an electromagnetic spectrum from a portion of the complex.

59. The method of claim 56, further comprising de-convoluting the electromagnetic spectrum by a computer implemented system to identify the reporter and providing analyte information based on the identity of the reporter.

60. The method of claim 56, wherein the electromagnetic spectrum is a Raman spectrum and the reporter is a COIN or a magnetic COIN.

61. A method for analyte concentration comprising capturing an analyte in a solution by a magnetic COIN attached to a first binding partner to form a magnetic COIN-containing complex comprising the analyte and the magnetic COIN attached to the first binding partner, and concentrating the magnetic COIN-containing complex in a magnetic field to form a concentrate comprising the magnetic COIN-containing complex.

62. The method of claim 61, further comprising removing the concentrate from the solution and placing the concentrate on surface comprising a second binding partner.

63. The method of claim 62, further comprising forming a sandwich layer comprising a binding complex comprising the second binding partner, the analyte, the first binding partner and the magnetic COIN.

64. The method of claim 63, further comprising detecting a Raman signal from the magnetic COIN by a Raman spectroscope.

65. The method of claim 64, further comprising de-convoluting the Raman signal to identify the analyte.

66. An analyte separation method comprising capturing a first analyte in a solution by a magnetic COIN attached to a first binding partner to form a magnetic COIN-containing complex comprising the first analyte and the magnetic COIN attached to the first binding partner, capturing a second analyte in the solution by a non-magnetic COIN attached to a second binding partner to form a non-magnetic COIN-containing complex comprising the second analyte and the non-magnetic COIN attached to the second binding partner, and grouping the magnetic COIN-containing complex separate from the non-magnetic COIN-containing complex on a surface of a microcoil by generating a local magnetic field on the surface of the microcoil.

67. The method of claim 66, further comprising controlling the current passing though the microcoil to control a strength of the local magnetic field on the surface of the microcoil.

68. The method of claim 66, further comprising releasing the magnetic COIN-containing complex from the surface of the microcoil.

69-70. -70. (canceled)