Systems and Methods for Evaluating Enzyme Competency
First Claim
1. A method for real-time, point of care determination of enzymatic system competency, the method comprising the steps of:
- a) administering at least one parent molecular entity to a patient for whom competency for at least one enzymatic system is to be determined, wherein at least one target marker is generated from said at least one parent molecular entity;
c) exposing a sensor to a bodily fluid from the patient, wherein said sensor can distinguish, detect, and quantify the target marker in the bodily fluid; and
d) based on the presence of the target marker, establishing whether the patient exhibits competency for the at least one enzymatic system.
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Accused Products
Abstract
The present invention provides systems and methods for determining enzymatic competency, which is important in determining whether a patient may suffer an adverse drug reaction, has a disease associated with defects in specific enzymatic function, and/or has an enzyme defect that is likely to cause pathophysiology. As contemplated herein, a parent molecular entity is administered to a patient in whom enzymatic competency is to be determined. A sample of the patient'"'"'s bodily fluid is exposed to a sensor of the invention to distinguish, detect, and quantify a detectable entity in the bodily fluid. Sensor-acquired data regarding the detectable entity is used to determine enzymatic competency. Preferably, a sample of a patient'"'"'s exhaled breath is collected and exposed to the sensor of the invention. Types of sensor systems of the invention include, but are not limited to, surface resonance arrays; microelectromechanical sensors (such as microcantilever-based technology); molecularly imprinted polymer sensors; amplifying fluorescent sensor technology; aptamer-based sensor technology; SAW sensors; infrared sensors; fuel cells; chemical reactors; and pH sensitive sensors.
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Citations
38 Claims
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1. A method for real-time, point of care determination of enzymatic system competency, the method comprising the steps of:
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a) administering at least one parent molecular entity to a patient for whom competency for at least one enzymatic system is to be determined, wherein at least one target marker is generated from said at least one parent molecular entity; c) exposing a sensor to a bodily fluid from the patient, wherein said sensor can distinguish, detect, and quantify the target marker in the bodily fluid; and d) based on the presence of the target marker, establishing whether the patient exhibits competency for the at least one enzymatic system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A system for real-time, point of care determination of enzymatic system competency, said system comprising:
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a) at least one parent molecular entity, wherein at least one target marker is generated from the at least one parent molecular entity; b) a sensor for detecting said target marker; and c) a processor configured to store and analyze information provided by the sensor. - View Dependent Claims (19, 20, 21, 22, 23, 25, 26)
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24. The system of claim 24, wherein the analogue of verapamil is N-(2,2,2-trifluoroethyl)norverapamil.
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27. A kit for point-of-care assessment of a patient'"'"'s enzymatic competency to at least one parent molecular entity, said kit comprising:
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a) a sample of said parent molecular entity, from which at least one target marker is generated; and b) at least one sensor. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36)
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37. A method for assessing potential patient organ suitability for transplantation comprising:
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a) administering at least one parent molecular entity to a patient for whom competency for at least one enzymatic system in an organ is to be determined, wherein at least one target marker is generated from said at least one parent molecular entity; c) exposing a sensor to a bodily fluid from the patient, wherein said sensor can distinguish, detect, and quantify the target marker in the bodily fluid; and d) based on the presence of the target marker, establishing whether the patient exhibits competency for the at least one enzymatic system and whether the organ is suitable for transplantation.
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38. A method for in vitro assessment of enzyme function comprising:
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a) administering in a closed container at least one parent molecular entity to a sample comprising at least one enzyme, wherein activity for the at least one enzyme is to be determined, wherein at least one target marker is generated from said at least one parent molecular entity, and wherein the closed container comprises a headspace; c) exposing a sensor to the headspace, wherein said sensor can distinguish, detect, and quantify the target marker in the headspace; and d) based on the presence of the target marker, establishing whether the at least one enzyme is active.
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Specification