MICROPLATE AND METHODS OF USING THE SAME

• US 20090010388A1
• Filed: 06/05/2008
• Published: 01/08/2009
• Est. Priority Date: 06/06/2007
• Status: Abandoned Application

• Current Assignee: Aptuit Limited
• Original Assignee: Aptuit Limited
• Inventors: STAHLY, BARBARA C., Wernicki, Alice
• Application Number:
  US12/133,639
• Publication Number:
  US 20090010388A1
• Time in Patent Office: Days
• US Class Current: 378/79
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 23/20 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials

Abstract

Microplates having a body defining a plurality of wells, wherein the wells have at least one bottom portion configured to allow the sample to be analyzed by various methods in situ with minimized background interference in the data obtained, and methods of using the same, are disclosed. The improved microplate may also have wells having certain shapes and/or sizes to allow the sample to be analyzed with minimized background interference in the data obtained. A system for obtaining high-quality XRPD or Raman data is also disclosed.

Claims

1. A microplate comprising:

a body defining a plurality of wells, each of the wells having at least one bottom portion configured to allow for transmission of X-rays during X-ray powder diffraction analysis with minimized background interference in the X-ray powder diffraction data.

2. The microplate of claim 1, wherein the at least one bottom portion has a thickness ranging from about 1 micron to about 1 millimeter.

3. The microplate of claim 2, wherein the at least one bottom portion has a thickness of about 37.5 microns.

4. The microplate of claim 1, wherein the at least one bottom portion comprises polypropylene.

5. The microplate of claim 4, wherein the at least one bottom portion comprises a polypropylene film.

6. The microplate of claim 5, wherein the film is heat-sealed to the body.

7. The microplate of claim 1, wherein the wells have a shape chosen from conical, cylindrical, frustoconical, or any combination thereof.

8. A microplate comprising:

a body defining a plurality of wells, each of the wells having at least one bottom portion,

wherein the at least one bottom portion comprises polypropylene and has a thickness ranging from about 1 micron to about 1 millimeter.

9. The microplate according to claim 8, wherein the at least one bottom portion has a thickness of about 37.5 microns.

10. The microplate according to claim 8, wherein the at least one bottom portion comprises a film of polypropylene which is heat-sealed onto the body.

11. A method of reducing background interference in X-ray powder diffraction data, the method comprising:

obtaining the X-ray powder diffraction data on a sample in a microplate comprising a body defining a plurality of wells, wherein the wells have at least one bottom portion, and wherein the at least one bottom portion is configured to allow for transmission of X-rays with minimized background interference in the X-ray powder diffraction data.

12. The method according to claim 11, wherein the at least one bottom portion has a thickness ranging from about 1 micron to about 1 millimeter.

13. The method according to claim 12, wherein the at least one bottom portion has a thickness of about 37.5 microns.

14. The method according to claim 11, wherein the at least one bottom portion comprises polypropylene.

15. The method according to claim 11, wherein the wells have a shape chosen from conical, cylindrical, frustoconical, or any combination thereof.

16. A method of reducing background intereference in X-ray powder diffraction data, the method comprising:

obtaining the X-ray powder diffraction data on a sample in a microplate wherein the microplate comprises a body defining a plurality of wells, wherein the wells have at east one bottom portion, and wherein the at least one bottom portion comprises polypropylene and has a thickness ranging from about 1 micron to about 1 millimeter.

17. The method according to claim 16, wherein the at least one bottom portion has a thickness of about 37.5 microns.

18. The method according to claim 16, wherein the at least one bottom portion comprises a film of polypropylene which is heat-sealed onto the body.

19. A method of obtaining improved X-ray powder diffraction data on a sample comprising obtaining the X-ray powder diffraction data on a sample in a microplate wherein the microplate comprises a body defining a plurality of wells, wherein the wells have at least one bottom portion, and wherein the at least one bottom portion is configured to allow for transmission of X-rays with minimized background interference in the X-ray powder diffraction data.

20. The method according to claim 19, wherein the at least one bottom portion has a thickness ranging from about 1 micron to about 1 millimeter.

21. The method according to claim 20, wherein the at least one bottom portion has a thickness of about 37.5 microns.

22. The method according to claim 19, wherein the at least one bottom portion comprises polypropylene.

23. The method according to claim 19, wherein the wells have a shape chosen from conical, cylindrical, frustoconical, or any combination thereof.

24. A system for obtaining improved X-ray powder diffraction data on a sample comprising an X-ray diffractometer and a microplate comprising a body defining a plurality of wells,

wherein the wells have at least one bottom portion,

wherein the at least one bottom portion is configured to allow for transmission of X-rays with minimized background interference in the X-ray powder diffraction data,

wherein the X-ray diffractometer comprises a plate holder,

and wherein the microplate is loaded onto the plate holder and X-rays are transmitted through the sample and the bottom portion with minimized background interference from the at least one bottom portion.

25. A method of reducing background interference in Raman data, the method comprising:

obtaining the Raman data on a sample in a microplate comprising a body defining a plurality of wells, wherein the wells have at least one bottom portion, and wherein the at least one bottom portion is configured to allow for transmission of Raman laser light with minimized background interference in the Raman data.

26. The method according to claim 25, wherein the at least one bottom portion has a thickness ranging from about 1 micron to about 1 millimeter.

27. The method according to claim 26, wherein the at least one bottom portion has a thickness of about 37.5 microns.

28. The method according to claim 25, wherein the at least one bottom portion comprises polypropylene.

29. The method according to claim 25, wherein the wells have a shape chosen from conical, cylindrical, frustoconical, or any combination thereof.

30. A method of reducing background interference in Raman data, the method comprising:

obtaining the Raman data on a sample in a microplate wherein the microplate comprises a body defining a plurality of wells, wherein the wells have at least one bottom portion, and wherein the at least one bottom portion comprises polypropylene and has a thickness ranging from about 1 micron to about 1 millimeter.

31. The method according to claim 30, wherein the at least one bottom portion has a thickness of about 37.5 microns.

32. The method according to claim 30 wherein the at least one bottom portion comprises a film of polypropylene which is heat-sealed onto the body.

33. A method of obtaining improved Raman data on a sample comprising obtaining the Raman data on a sample in a microplate wherein the microplate comprises a body defining a plurality of wells, wherein the wells have at least one bottom portion, and wherein the at least one bottom portion is configured to allow for transmission of Raman laser light with minimized background interference in the Raman data.

34. The method according to claim 33, wherein the at least one bottom portion has a thickness ranging from about 1 micron to about 1 millimeter.

35. The method according to claim 34, wherein the at least one bottom portion has a thickness of about 37.5 microns.

36. The method according to claim 33, wherein the at least one bottom portion comprises polypropylene.

37. The method according to claim 33, wherein the wells have a shape chosen from conical, cylindrical, frustoconical, or any combination thereof.

38. A system for obtaining improved Raman data on a sample comprising a Raman spectrometer and a microplate comprising a body defining a plurality of wells,

wherein the wells have at least one bottom portion,

wherein the at least one bottom portion is configured to allow for transmission of Raman laser light with minimized background interference in the Raman data,

wherein the Raman spectrometer comprises a plate holder,

and wherein the microplate is loaded onto the plate holder and Raman laser light is transmitted through the sample and the bottom portion with minimized background interference from the at least one bottom portion.