Embryonic Stem Cell Derivatives, and Methods of Making and Using the Same
First Claim
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16. A method for producing a reagent comprising an in vitro differentiated cell, the method comprising:
- (a) providing an embryonic stem (ES) cell or a pre-embryoid body cell; and
(b) differentiating the ES cell or the pre-embryoid body cell in vitro, wherein the differentiating leads to the ES cell or pre-embryoid body cell;
(i) expressing a marker associated with a differentiated cell of interest;
(ii) differentiating into a cell type of interest;
or(iii) combinations thereof.
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Abstract
The presently disclosed subject matter provides embryonic stem (ES) cell derivatives that can be employed in therapeutic methods. Also provided are methods for generating and using the ES cell derivatives.
19 Citations
27 Claims
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16. A method for producing a reagent comprising an in vitro differentiated cell, the method comprising:
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(a) providing an embryonic stem (ES) cell or a pre-embryoid body cell; and (b) differentiating the ES cell or the pre-embryoid body cell in vitro, wherein the differentiating leads to the ES cell or pre-embryoid body cell; (i) expressing a marker associated with a differentiated cell of interest; (ii) differentiating into a cell type of interest;
or(iii) combinations thereof. - View Dependent Claims (17, 18, 19, 20)
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21. A method for generating a putative endoderm precursor cell (PEP) from an embryonic stem cell, the method comprising:
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(a) culturing the embryonic stem cell in the absence of feeder cells and LIF; and (b) exposing the embryonic stem cell to acidic fibroblast growth factor in an amount and for a time sufficient to provide reduced expression of Oct4 and increased expression of an mRNA selected from the group consisting of α
-fetoprotein, Gata4, albumin, FoxA2 (HNF-β
3), and Sox 17α
in the embryonic stem cell, whereby a PEP is generated. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 22, 23, 24, 25, 26, 27)
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26-1. The cell of claim 25, where the cell is capable of engraftment of an endoderm-derived tissue of a subject when administered into the endoderm-derived tissue.
Specification