Dna Crosslinking for Primer Extension Assays
First Claim
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1. A method of identifying nucleotides in a nucleic acid template comprising contacting a primer with a nucleic acid template, crosslinking the primer to the template, and extending the primer under conditions that denature undesirable secondary structures and detecting extension of the primer, thereby identifying one or more nucleotides in the template.
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Abstract
Provided herein are compositions and methods for inhibiting false signals associated with mispriming in primer extension assays.
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Citations
23 Claims
- 1. A method of identifying nucleotides in a nucleic acid template comprising contacting a primer with a nucleic acid template, crosslinking the primer to the template, and extending the primer under conditions that denature undesirable secondary structures and detecting extension of the primer, thereby identifying one or more nucleotides in the template.
Specification