Method For Making Microsensor Arrays For Detecting Analytes

• US 20090011945A1
• Filed: 12/17/2007
• Published: 01/08/2009
• Est. Priority Date: 07/28/1999
• Status: Abandoned Application

• Current Assignee: The Research Foundation for The State University of New York (State University of New York)
• Original Assignee: The Research Foundation for The State University of New York (State University of New York)
• Inventors: Cho, Eun Jeong, Bright, Frank V.
• Application Number:
  US11/957,915
• Publication Number:
  US 20090011945A1
• Time in Patent Office: Days
• US Class Current: 506/7
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

The present invention provides a method and a device for high-throughput, simultaneous, and continuous detection of one or more analytes using a pin-printed chemical sensor array. Chemical sensors comprising [Ru(4,7-diphenyl-1,10-phenanthroline)₃]²⁺, glucose oxidase, and fluorescein sequestered in sol-gel-derived-glass have been used. Examples of analytes detected using the present method include O₂, glucose, and protons. Gas-phase and liquid-phase analytes have been detected using the present method. In addition, analytes contained in an aerosol have been detected.

Claims

1. ) A method for high-throughput continuous detection of one or more analytes in a plurality of test samples comprising the steps of:

a) providing a device comprising;

i) a substrate;

ii) an array of pin-printed spots printed on the substrate, each pin-printed spot having a holding material and chemical sensor sequestered within the holding material, wherein the holding material is a sol-gel derived glass;

iii) a detector for continuously recording a signal comprising emitted light from each pin-printed spot;

b) contacting the array with one or more samples containing one or more analytes;

c) irradiating the array with an electromagnetic radiation of 200 to 900 nm; and

d) detecting the signal from each pin-printed spot as a function of time.

2. ) The method of claim 1, wherein at least two pin-printed spots in the array have different chemical sensors.

3. ) The method of claim 1, wherein the pin-printed spots are printed on the substrate using a using a sol-gel processing comprising tetraethoxysilane, trimethoxysilane, n-propyltrimethoxysilane or combinations thereof.

4. ) The method of claim 1, wherein the sol-gel-derived glass is xerogel.

5. ) The method of claim 1, wherein the sol-gel-derived glass is doped with a polymer.

6. ) The method of claim 5, wherein the polymer an organic polymer selected from the group consisting of polyethylene glycol and Pluronic P104 and combinations thereof.

7. ) The method of claim 1, wherein the step of irradiating the array is carried out by an electromagnetic radiation generator integrated within the device.

8. ) The method of claim 7, wherein the device is a light-emitting diode.

9. ) The method of claim 1, wherein the chemical sensor is selected from the group consisting of Ru[(4,7-diphenyl-1,10-phenanthroline)₃]²⁺, fluorescein-linked dextran, and glucose oxidase.

10. ) The method of claim 1, wherein the analyte is selected from the group consisting of O₂, glucose, protons and combinations thereof.

11. ) The method of claim 10, wherein the O₂ is in the gas phase.

12. ) The method of claim 10, wherein the O₂ is in solution.

13. ) The method of claim 10, wherein glucose is in solution.

14. ) The method of claim 10, wherein protons are in solution.

15. ) The method of claim 1, wherein the sample is in the form of an aerosol.

16. ) The method of claim 15, wherein the analyte in the sample is glucose.

17. ) The method of claim 1, wherein the analyte can be continuously detected for a period of at least 3 hours.

18. ) The method of claim 1, wherein the analyte can be continuously detected for a period of at least 42 days.

19. ) The method of claim 1, wherein the analyte can be continuously detected for a period of at least 18 months.