Compound Screening Using Cardiomyocytes
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Abstract
This invention provides populations human cells of the cardiomyocyte lineage. The cells are obtained by causing cultures of pluripotent stem cells to differentiate in vitro, and then harvesting cells with certain phenotypic features. Differentiated cells bear cell surface and morphologic markers characteristic of cardiomyocytes, and a proportion of them undergo spontaneous periodic contraction. Highly enriched populations of cardiomyocytes and their replicating precursors can be obtained, suitable for use in a variety of applications, such as drug screening and therapy for cardiac disease.
59 Citations
35 Claims
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1-20. -20. (canceled)
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21. A method of screening a compound for an effect on a cardiomyocyte in cell culture, comprising:
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a) contacting the cell culture with the compound; and b) observing a change in the cardiomyocytes in the cell culture, wherein the cell culture comprises 3% or more cardiomyocytes that are the progeny of a non-malignant in vitro cell culture comprising cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28)
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29. A method of screening a compound for an effect on a cell in cell culture, comprising:
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a. contacting the cell culture with a compound; and b. observing a change in a myosin heavy chain expressing cells in the cell culture, wherein the cell culture comprises 3% or more cells that express myosin heavy chain and are the progeny of a non-malignant in vitro cell culture comprising cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81. - View Dependent Claims (30, 31, 32, 33, 34, 35)
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Specification