MODIFIED GREEN FLUORESCENT PROTEINS AND METHODS FOR USING SAME

• US 20090017535A1
• Filed: 07/24/2008
• Published: 01/15/2009
• Est. Priority Date: 11/04/2005
• Status: Active Grant

• Current Assignee: Evrogen Joint Stock Company
• Original Assignee: Evrogen Joint Stock Company
• Inventors: Lukyanov, Sergey A.
• Granted Patent: US 7,605,230 B2
• Time in Patent Office: Days
• US Class Current: 435/348
• CPC Class Codes: C07K 14/43595 from coelenteratae, e.g. medusae

Abstract

The present invention provides nucleic acid molecules encoding mutant fluorescent proteins as well as proteins encoded by these nucleic acids. In addition, host-cells, stable cell lines and transgenic organisms comprising the above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells, or cell organelles.

Claims

1. An isolated genetically engineered fluorescent protein that comprises amino acid sequence with at least 97% identity with the amino acid sequence of SEQ ID NO:

18.

2. The fluorescent protein of claim 1, wherein the amino acid sequence of the genetically engineered fluorescent protein comprises one or more amino acid substitutions selected from the group consisting of K3G, E6D, T9A, P58T, F99L, F99H, M128K, M128E, I136M, Y151H, N144S, K162E, K156M, T214A, G228C, G228S, and K238R.

3. The fluorescent protein of claim 1, wherein the genetically engineered fluorescent protein comprises an amino acid sequence of SEQ ID NO:

18.

4. A fusion protein comprising the fluorescent protein of claim 1.

5. A host cell comprising fluorescent protein of claim 1.

6. A host cell comprising fusion protein of claim 4.