NANOGRID ROLLING CIRCLE DNA SEQUENCING
First Claim
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1. A method of sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size comprising:
- synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region;
immobilizing the concatemer to the specific region to make an immobilized concatemer; and
sequencing the immobilized concatemer.
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Abstract
The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
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Citations
19 Claims
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1. A method of sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size comprising:
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synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region; immobilizing the concatemer to the specific region to make an immobilized concatemer; and sequencing the immobilized concatemer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size comprising:
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synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region; immobilizing the concatemer to the specific region to make an immobilized concatemer; clonally amplifying the immobilized concatemer to make a clonally amplified concatemer; and sequencing the clonally amplified concatemer. - View Dependent Claims (17, 18)
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19. A method of sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size of less than 300 nanometers comprising:
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synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter of greater than 300 nanometers; immobilizing the concatemer to the specific region to make an immobilized concatemer; and sequencing the immobilized concatemer.
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Specification