Method for maintaining pluripotency of stem/progenitor cells
First Claim
1. A method for maintaining pluripotency and/or self-renewing characteristics of stem/progenitor cells, the method comprising:
- (a) contacting at least two transcription factors, or a functional fragment thereof, with the promoter region of the nanog gene, wherein one of the at least two transcription factors is selected from the POU- and homeo-domain-containing transcription factors, and wherein another of the at least two transcription factors is selected from the HMG domain-containing transcription factors; and
(b) allowing said at least two transcription factors to form a complex with a specific binding element within the nanog promoter, said complex regulating nanog gene expression by mediating transcriptional activation.
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Abstract
The present invention relates to a method for maintaining pluripotency and/or self-renewing characteristics of stem/progenitor cells. The invention also relates to a method for modulating gene expression in a cell. The methods include contacting at least two transcription factors, or a functional fragment thereof, with the promoter region of the nanog gene. One of the at least two transcription factors is selected from the POU- and homeo-domain-containing transcription factors. Another of the at least two transcription factors is selected from the HMG domain-containing transcription factors. The method further comprises allowing the at least two transcription factors to form a complex with a specific binding element within the nanog promoter. The complex thus formed regulates nanog gene expression by mediating transcriptional activation.
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Citations
44 Claims
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1. A method for maintaining pluripotency and/or self-renewing characteristics of stem/progenitor cells, the method comprising:
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(a) contacting at least two transcription factors, or a functional fragment thereof, with the promoter region of the nanog gene, wherein one of the at least two transcription factors is selected from the POU- and homeo-domain-containing transcription factors, and wherein another of the at least two transcription factors is selected from the HMG domain-containing transcription factors; and (b) allowing said at least two transcription factors to form a complex with a specific binding element within the nanog promoter, said complex regulating nanog gene expression by mediating transcriptional activation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 42)
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20. A method for modulating gene expression in a cell, the method comprising:
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(a) contacting at least two transcription factors or a functional fragment thereof with the promoter region of the nanog gene, wherein one of the at least two transcription factors is selected from the POU- and homeo-domain-containing transcription factors, and wherein another of the at least two transcription factors is selected from the HMG domain-containing transcription factors; and (b) allowing said at least two transcription factors to form a complex with a specific binding element within the nanog promoter, said complex regulating gene expression by mediating transcriptional activation. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 43, 44)
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Specification