Biosensor Chip and Biosensor Chip Production Method

• US 20090020420A1
• Filed: 10/16/2006
• Published: 01/22/2009
• Est. Priority Date: 10/17/2005
• Status: Abandoned Application

• Current Assignee: National Institute of Advanced Industrial Science and Technology (Government of Japan), Sumitomo Electric Industries Limited (Sumitomo Corporation)
• Original Assignee: Sumitomo Electric Industries Limited (Sumitomo Corporation)
• Inventors: Nakamura, Hideaki, Gotoh, Masao, Karube, Isao, Ishikawa, Tomoko, Hosoya, Toshifumi, Kitamura, Takahiko, Kaimori, Shingo, Ichino, Moriyasu
• Application Number:
  US11/921,166
• Publication Number:
  US 20090020420A1
• Time in Patent Office: Days
• US Class Current: 204/403.140
• CPC Class Codes: C12Q 1/002 Electrode membranes

Abstract

It is intended to provide a biosensor chip capable of rapid and correct measurement, including a reaction chamber that enables a measurement of a very small amount of a measurement sample and has a small capacity and potassium ferricyanide having a very small crystal particle size disposed in the reaction chamber.

In a biosensor chip 1, electrodes 3 and 4 are disposed on the lower substrate 2, and lower spacer 5 (7 and 8) is adhered on the electrodes 3 and 4. An upper spacer 11 (12 and 13) is adhered to an upper substrate 15, and a lower spacer 5 is attached to the upper spacer 11 by and adhesive agent 10. A lower groove 9 is formed between long and short lower spacers 7 and 8, and an upper groove 14 is formed between long and short spacers 12 and 13, so that a reaction chamber 17 is formed by the upper and lower grooves 9 and 14. A capacity of the reaction chamber is 0.3 μL, and an enzyme 18 and potassium ferricyanide are disposed in the reaction chamber 17 in such a fashion as to oppose to each other with a gap being defined therebetween. A crystal particle diameter of the potassium ferricyanide is 100 μm or less, and amount of the potassium ferricyanide is V×0.1 mg or more when the capacity of the reaction chamber 17 is V.

Claims

1. A biosensor chip comprising:

upper and lower substrates,

at least two electrodes disposed on at least one of the upper and lower substrates, and

a reaction chamber for performing a chemical reaction, wherein

at least an enzyme and potassium ferricyanide are included as reagents to be placed in the reaction chamber;

a capacity of the potassium ferricyanide placed in the reaction chamber is V×

0.1 mg or more when a capacity of the reaction chamber is V μ

L; and

a maximum diameter of crystal particles of the potassium ferricyanide is 100 μ

m.

2. The biosensor chip according to claim 1, wherein the maximum diameter of the crystal particles of the potassium ferricyanide may preferably be 50 μ

m or less.

3. The biosensor chip according to claim 1, wherein

the enzyme and the potassium ferricyanide are placed in the reaction chamber with a gap being defined therebetween.

4. The biosensor chip according to claim 1, wherein

the upper and lower substrates are formed from one sheet, and

the one sheet is folded to form the upper and lower substrates.

5. A biosensor chip production method for producing a biosensor chip including upper and lower substrates, at least two electrodes disposed on at least one of the upper and lower substrates, and a reaction chamber for performing a chemical reaction, the method comprising:

a step of coating at least an enzyme and potassium ferricyanide in the reaction chamber, and

a step of freezing, heating, or mixing with a poor solvent the potassium ferricyanide in such a manner that a maximum diameter of crystal particles of the potassium ferricyanide becomes 100 μ

m or less and a capacity of the potassium ferricyanide placed in the reaction chamber becomes V×

0.1 mg or more when a capacity of the reaction chamber is V μ

L.

6. The biosensor production method according to claim 5, wherein

one sheet is folded to form the upper and lower substrates.