COMPLEMENTARY METABOLIZING ORGANISMS AND METHODS OF MAKING SAME
First Claim
1. A non-naturally occurring set of microbial organisms, comprising:
- at least a first constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize a first carbon substrate and having substantially impaired metabolic capacity for a second carbon substrate, andat least a second constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize said second carbon substrate and having substantially impaired metabolic capacity for said first carbon substrate,wherein a co-culture of said at least first and second CMOs exhibit simultaneous metabolism of a mixture comprising said first and second carbon substrates compared to either CMO alone.
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Abstract
The invention provides a non-naturally occurring set of microbial organisms. The set of organisms includes: at least a first constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize a first carbon substrate and having substantially impaired metabolic capacity for a second carbon substrate, and at least a second constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize the second carbon substrate and having substantially impaired metabolic capacity for the first carbon substrate, wherein a co-culture of the at least first and second CMOs exhibit simultaneous metabolism of a mixture having the first and second carbon substrates compared to either CMO alone. Simultaneous metabolism of a mixture having first and second carbon substrates can include an enhanced rate of metabolism of the first and second substrates compared to either CMO alone. Also provided is a bioprocess for producing a chemical compound. The bioprocess includes co-culturing a non-naturally occurring set of microbial organisms in a mixture having at least a first and a second carbon substrate under conditions sufficient for biosynthesis of a target chemical compound, the set of non-naturally occurring microbial organisms including: at least a first constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize the first carbon substrate and having substantially impaired metabolic capacity for the second carbon substrate, and at least a second constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize the second carbon substrate and having substantially impaired metabolic capacity for the first carbon substrate, wherein a co-culture of the at least first and second CMOs exhibit simultaneous metabolism of a mixture having the first and second carbon substrates compared to either CMO alone. Simultaneous metabolism of a mixture having first and second carbon substrates can include an enhanced rate of metabolism of the first and second substrates compared to either CMO alone.
86 Citations
31 Claims
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1. A non-naturally occurring set of microbial organisms, comprising:
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at least a first constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize a first carbon substrate and having substantially impaired metabolic capacity for a second carbon substrate, and at least a second constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize said second carbon substrate and having substantially impaired metabolic capacity for said first carbon substrate, wherein a co-culture of said at least first and second CMOs exhibit simultaneous metabolism of a mixture comprising said first and second carbon substrates compared to either CMO alone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A bioprocess for producing a chemical compound, comprising co-culturing a non-naturally occurring set of microbial organisms in a mixture comprising at least a first and a second carbon substrate under conditions sufficient for biosynthesis of a target chemical compound, said set of non-naturally occurring microbial organisms comprising:
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at least a first constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize said first carbon substrate and having substantially impaired metabolic capacity for said second carbon substrate, and at least a second constituent complementary metabolizing organism (CMO) exhibiting the ability to metabolize said second carbon substrate and having substantially impaired metabolic capacity for said first carbon substrate, wherein a co-culture of said at least first and second CMOs exhibit simultaneous metabolism of a mixture comprising said first and second carbon substrates compared to either CMO alone. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification