Applications with and Methods for Producing Selected Interstrand Cross-Links in Nucleic Acids

• US 20090023910A1
• Filed: 08/25/2008
• Published: 01/22/2009
• Est. Priority Date: 12/03/1998
• Status: Abandoned Application

• Current Assignee: Kreatech Biotechnology BV (Danaher Corporation)
• Original Assignee: Kreatech Biotechnology BV (Danaher Corporation)
• Inventors: Houthoff, Hendrik-Jan, van Gijlswijk, R.P.M., Tanke, Hendrikus Johannes, Volkers, Herman, Raap, Anton Klaas, Heetebrij, Robert
• Application Number:
  US12/197,590
• Publication Number:
  US 20090023910A1
• Time in Patent Office: Days
• US Class Current: 536/25.300
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6832 Enhancement of hybridisation reaction
• CPC Class Codes: C12Q 1/6841 In situ hybridisation
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2523/101 Crosslinking agents, e.g. psoralen
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid
• CPC Class Codes: C12Q 2525/151 repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
• CPC Class Codes: C12Q 2525/186 incorporating a non-extendable or blocking moiety
• CPC Class Codes: C12Q 2563/137 Metal/ion, e.g. metal label
• CPC Class Codes: C12Q 2565/102 Multiple non-interacting labels

Abstract

The invention provides a method for stably cross-linking a first nucleic acid sequence selected for being stably cross-linked in the presence of a second nucleic acid sequence selected for not being stably cross-linked in a nucleic acid molecule. The method comprises providing a nucleic acid sequence that is complementary to the first nucleic acid sequence; hybridizing the first nucleic acid sequence to its complementary sequence; and stably cross-linking the first nucleic acid sequence to the complementary sequence.

Claims

1-26. -26. (canceled)

27. A method for preventing hybridization of a first chromosomal nucleic acid sequence, while permitting hybridization of a second chromosomal nucleic acid sequence, the method comprising:

(a) providing a nucleic acid sequence that is complementary to a first chromosomal nucleic acid sequence wherein the complementary sequence or the first chromosomal nucleic acid sequence or both comprise a cross-linking agent, the cross-linking agent being capable of cross-linking the complementary sequence to the first chromosomal nucleic acid sequence; and

(b) hybridizing the first chromosomal nucleic acid sequence to its complementary sequence in the presence of the second chromosomal nucleic acid sequence;

thereby cross-linking the complementary sequence to the first chromosomal nucleic acid sequence;

whereby the cross-linked first chromosomal nucleic acid sequence is prevented from further hybridizing and the second chromosomal nucleic acid sequences is permitted to hybridize.

28. The method according to claim 27, wherein the complementary nucleic acid sequence is DNA, RNA, or PNA.

29. The method according to claim 27, wherein the first chromosomal nucleic acid sequence is DNA.

30. The method according to claim 27, wherein the cross-linking agent comprises a transition metal.

31. The method according to claim 30, wherein the transition metal is platinum.

32. The method according to claim 27, wherein the cross-linking agent is trans-dichlorodiammineplatinum.