Cryoprotective Compositions and Methods of Using Same

• US 20090029340A1
• Filed: 01/04/2007
• Published: 01/29/2009
• Est. Priority Date: 01/04/2006
• Status: Abandoned Application

• Current Assignee: Do-Coop Technologies Ltd.
• Original Assignee: Do-Coop Technologies Ltd.
• Inventors: Gabbai, Eran
• Application Number:
  US12/087,429
• Publication Number:
  US 20090029340A1
• Time in Patent Office: Days
• US Class Current: 435/1.300
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0221 Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
• CPC Class Codes: A61P 31/02 Local antiseptics
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C12Q 1/6851 Quantitative amplification
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles

Abstract

A cryoprotective composition which comprises nanostructures, liquid and at least one cryoprotective agent is provided.

Claims

1. A cryoprotective composition comprising nanostructures, liquid and at least one cryoprotective agent.

20. The cryoprotective composition of claim 1, wherein said nanostructures are formulated from hydroxyapatite.

21. The cryoprotective composition of claim 1, comprising less than 10% by volume glycerol.

22. The cryoprotective composition of claim 1 being devoid of glycerol.

24. The cryoprotective composition of claim 1 further comprising a stabilizer.

2. A method of cryopreserving cellular matter comprising:

(a) contacting the cellular matter with a composition comprising nanostructures and a liquid; and

(b) subjecting the cellular matter to a cryopreserving temperature, thereby cryopreserving the cellular matter.

6. The method of claim 2, wherein the cryoprotective composition further comprises at least one cryoprotective agent.

29. The method of claim 2, wherein said cellular matter is selected from the group comprising a body fluid, a cell culture, a cell suspension, a cell matrix, a tissue, an organ and an organism.

30. The method of claim 29, wherein said body fluid is semen.

36. The method of claim 2, wherein said cellular matter is selected from the group consisting of a stem cell, a sperm, an egg and an embryo.

38. The method of claim 2, further comprising conditioning the cellular matter prior to step (a).

40. The method of claim 2, wherein step (a) and step (b) are performed simultaneously.

3. A method of recovering cryopreserved cellular matter comprising:

(a) contacting cellular matter with a composition comprising nanostructures and a liquid;

(b) subjecting the cellular matter to a cryopreserving temperature to generate cryopreserved cellular matter;

(c) thawing said cryopreserved cellular matter; and

(d) removing said composition;

thereby recovering cryopreserved cellular matter.

41. The method of claim 3 wherein said cryopreserving temperature is less than about −

80°

C.

43. The method of claim 3, wherein said cellular matter is selected from the group comprising a body fluid, a cell culture, a cell suspension, a cell matrix, a tissue, an organ and an organism.

44. The method of claim 43, wherein said body fluid is semen.

45. The method of claim 3, wherein said cellular matter is selected from the group consisting of a stem cell, a sperm, an egg and an embryo.

4. (canceled)

5. A cryopreservation container comprising nanostructures and a liquid.

42. The cryopreservation container of claim 5, further comprising at least one cryoprotective agent.

7-19. -19. (canceled)

23. (canceled)

25-28. -28. (canceled)

31-35. -35. (canceled)

37. (canceled)

39. (canceled)