PROCESS FOR THE PRODUCTION OF BETA-LYSINE

• US 20090029425A1
• Filed: 03/07/2007
• Published: 01/29/2009
• Est. Priority Date: 03/09/2006
• Status: Abandoned Application

• Current Assignee: BASF SE
• Original Assignee: BASF SE
• Inventors: Jeong, Weo Kyu, Zelder, Oskar, Schroder, Hartwig, Klopprogge, Corinna, Herold, Andrea
• Application Number:
  US12/281,789
• Publication Number:
  US 20090029425A1
• Time in Patent Office: Days
• US Class Current: 435/115
• CPC Class Codes: C12P 13/005 Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
• CPC Class Codes: C12P 13/02 Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes peptides C12P21/00 or C07K
• CPC Class Codes: C12P 13/08 Lysine; Diaminopimelic acid; Threonine; Valine

Abstract

Process for the production of -lysine by constructing a recombinant microorganism which has a deregulated lysine 2,3-aminomutase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diamino-pimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

Claims

1. (canceled)

2. (canceled)

3. (canceled)

4. (canceled)

5. (canceled)

6. (canceled)

7. (canceled)

8. (canceled)

9. (canceled)

10. A process for the production of Compound A, wherein Compound A is β

-lysine, β

-amino-ε

-caprolactam, ε

-caprolactam or ε

-aminocaproic acid, and wherein the process comprises constructing a recombinant microorganism comprising a deregulated lysine 2,3-aminomutase gene and at least one deregulated gene selected from the group (i) consisting of genes encoding aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, and methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i), at least a second gene (i) other than aspartokinase is deregulated; and

cultivating the microorganism.

11. The process of claim 10, wherein compound A is β

-lysine.

12. The process of claim 11, wherein the microorganism belongs to the genus Corynebacterium.

13. The process of claim 11, wherein the microorganism is Corynebacterium glutamicum.

14. The process of claim 11, wherein the deregulated lysine-2,3-aminomutase gene encodes a lysine-2,3-aminomutase heterologous to the microorganism.

15. The process of claim 11, wherein the recombinant microorganism comprises a lysine-2,3-aminomutase gene from Clostridium, Bacillus or Escherichia.

16. The process of claim 11, wherein the lysine-2,3-aminomutase comprises a polypeptide sequence of Clostridium subterminale, Bacillus subtilis or Escherichia coli lysine-2,3-aminomutase or a polypeptide sequence with a lysine 2,3-aminomutase activity which is at least 80% identical to the corresponding original polypeptide.

17. The process of claim 10, wherein Compound A is β

-amino-ε

-caprolactam.

18. The process of claim 10, wherein Compound A is ε

-caprolactam.

19. The process of claim 10, wherein Compound A is ε

-aminocaproic acid.