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Ultra High-Throughput Opti-Nanopore DNA Readout Platform

  • US 20090029477A1
  • Filed: 08/12/2005
  • Published: 01/29/2009
  • Est. Priority Date: 08/13/2004
  • Status: Active Grant
First Claim
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1. A method for analyzing one or more target polynucleotide molecules comprising:

  • (a) designing one or more Design Monomers using said target polynucleotide as a template, wherein an individual Design Monomer further corresponds to either a “

    0”

    or a “

    1”

    , wherein two individual Design Monomers correspond to a predetermined nucleotide base, and wherein said individual Design Monomer has one or more signal molecules;

    (b) forming one or more Design Polymers by ligating a plurality of said Design Monomers;

    (c) repeating steps (a) and (b) until one or more predetermined Design Polymers are constructed;

    (d) forming a Design Polymer—

    signal complex by admixing, under conditions suitable for hybridization, said Design Polymer with a signal molecule; and

    (e) subjecting said complex of (d) to analysis.

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