Ultra High-Throughput Opti-Nanopore DNA Readout Platform
First Claim
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1. A method for analyzing one or more target polynucleotide molecules comprising:
- (a) designing one or more Design Monomers using said target polynucleotide as a template, wherein an individual Design Monomer further corresponds to either a “
0”
or a “
1”
, wherein two individual Design Monomers correspond to a predetermined nucleotide base, and wherein said individual Design Monomer has one or more signal molecules;
(b) forming one or more Design Polymers by ligating a plurality of said Design Monomers;
(c) repeating steps (a) and (b) until one or more predetermined Design Polymers are constructed;
(d) forming a Design Polymer—
signal complex by admixing, under conditions suitable for hybridization, said Design Polymer with a signal molecule; and
(e) subjecting said complex of (d) to analysis.
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Abstract
Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises (1) the electrically controlled unzipping of DNA (or RNA) double strands, and (2) the readout of the molecule'"'"'s identity (or code) using one or more molecule signal detection.
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Citations
53 Claims
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1. A method for analyzing one or more target polynucleotide molecules comprising:
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(a) designing one or more Design Monomers using said target polynucleotide as a template, wherein an individual Design Monomer further corresponds to either a “
0”
or a “
1”
, wherein two individual Design Monomers correspond to a predetermined nucleotide base, and wherein said individual Design Monomer has one or more signal molecules;(b) forming one or more Design Polymers by ligating a plurality of said Design Monomers; (c) repeating steps (a) and (b) until one or more predetermined Design Polymers are constructed; (d) forming a Design Polymer—
signal complex by admixing, under conditions suitable for hybridization, said Design Polymer with a signal molecule; and(e) subjecting said complex of (d) to analysis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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53. A method for analyzing one or more target polynucleotide molecules comprising:
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(a) designing one or more Design Monomers using said target polynucleotide as a template, wherein an individual Design Monomer further corresponds to either a “
0”
or a “
1”
, wherein two individual Design Monomers correspond to a predetermined nucleotide base, and wherein said individual Design Monomer has one or more signal molecules;(b) forming one or more Design Polymers by ligating a plurality of said Design Monomers; (c) repeating steps (a) and (b) until one or more predetermined Design Polymers are constructed; (d) forming a Design Polymer—
signal complex by admixing, under conditions suitable for hybridization, said Design Polymer with a signal molecule;(e) introducing said complex of (d) to a nanopore system, wherein said complex is unzipped, and wherein a single-stranded portion of said Design Polymer enters into and through said nanopore system; and (f) analyzing said single-stranded Design Polymer of (e).
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Specification