Optical interrogation system and method for using same
First Claim
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1. An optical interrogation system comprising:
- a tunable laser for generating an optical beam;
a first fiber launch for receiving the optical beam generated by said tunable laser and then outputting a first optical beam;
a first lens for collimating the first optical beam outputted from said first fiber launch such that the collimated optical beam illuminates a first biosensor;
said first lens for receiving a first reflected optical beam from the first biosensor;
a first detector, located ahead or past a focal point of said first lens, for receiving a first defocused optical beam from said first lens; and
a data processing device for receiving one or more intensity spot patterns associated with the first defocused optical beam received at said first detector.
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Abstract
An optical interrogation system and a method are described herein that enable the interrogation of one or more biosensors which can be located within the wells of a microplate. In one embodiment, the optical interrogation system has a tunable laser, N-fiber launches, N-lenses and N-detectors that are set-up to interrogate N-biosensors. In another embodiment, the optical interrogation system has a tunable laser, N-fiber launches, N+1 lenses and N-detectors that are set-up to interrogate N-biosensors.
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Citations
30 Claims
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1. An optical interrogation system comprising:
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a tunable laser for generating an optical beam; a first fiber launch for receiving the optical beam generated by said tunable laser and then outputting a first optical beam; a first lens for collimating the first optical beam outputted from said first fiber launch such that the collimated optical beam illuminates a first biosensor; said first lens for receiving a first reflected optical beam from the first biosensor; a first detector, located ahead or past a focal point of said first lens, for receiving a first defocused optical beam from said first lens; and a data processing device for receiving one or more intensity spot patterns associated with the first defocused optical beam received at said first detector. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for interrogating one or more biosensors, said method comprising the steps of:
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emitting a first optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; using a first lens to collimate the first emitted optical beam such that the first collimated optical beam illuminates a first biosensor; using said first lens to receive a first reflected optical beam from the first biosensor; using a first detector to receive the first reflected optical beam from said first lens, where the first detector is positioned ahead or past a focal point of said first lens; and using a data processing device to receive a plurality of intensity spot patterns associated with the first reflected optical beam that was received at said first detector where each intensity spot pattern corresponds with one of the distinct wavelengths of the first emitted optical beam. - View Dependent Claims (8, 9, 10, 11, 12)
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13. An optical interrogation system comprising:
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a tunable laser for emitting an optical beam; a first fiber launch for receiving the optical beam emitted from said tunable laser and then outputting a first optical beam; a first lens for collimating the first optical beam outputted from said first fiber launch such that the collimated first optical beam illuminates a first biosensor; a second lens for receiving a first reflected optical beam from the first biosensor; a first detector for receiving the first reflected optical beam from said second lens; and a data processing device for receiving one or more images associated with the first reflected optical beam received at said first detector; a second fiber launch for receiving the optical beam generated by said tunable laser and then outputting a second optical beam; said second lens for collimating the second optical beam outputted from said second fiber launch such that the collimated second optical beam illuminates a second biosensor; a third lens for receiving a second reflected optical beam from the second biosensor; a second detector for receiving the second reflected optical beam from said third lens; and said data processing device for receiving one or more images associated with the second reflected optical beam received at said second detector. - View Dependent Claims (15, 19, 20, 21, 22, 23, 24)
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14. (canceled)
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16. A method for interrogating one or more biosensors, said method comprising the steps of:
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emitting a first optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; using a first lens to collimate the first optical beam such that the first collimated optical beam illuminates a first biosensor; using a second lens to receive a first reflected optical beam from the first biosensor; using a first detector to receive the first reflected optical beam from said second lens; and using a data processing device to receive a plurality of images associated with the first reflected optical beam that was received at said first detector where each image corresponds with one of the distinct wavelengths of the first emitted optical beams; emitting a second optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; using said second lens to collimate the second optical beam such that the second collimated optical beam illuminates a second biosensor; using a third lens to receive a second reflected optical beam from the second biosensor; using a second detector to receive the second reflected optical beam from said third lens; and using a data processing device to receive a plurality of images associated with the second reflected optical beam that was received at said second detector where each image corresponds with one of the distinct wavelengths of the second emitted optical beam. - View Dependent Claims (18, 25, 26, 27, 28, 29, 30)
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17. (canceled)
Specification