Tissue engineering support

• US 20090035858A1
• Filed: 07/30/2007
• Published: 02/05/2009
• Est. Priority Date: 07/30/2007
• Status: Active Grant

• Current Assignee: Sofradim Production
• Original Assignee: Sofradim Production
• Inventors: Bayon, Yves, Lefranc, Olivier, Gravagna, Philippe, Meneghin, Alfredo, Therin, Michel
• Granted Patent: US 8,198,087 B2
• Time in Patent Office: Days
• US Class Current: 435/398
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: C08L 89/06 derived from leather or skin , e.g. gelatin
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0661 Smooth muscle cells
• CPC Class Codes: D04B 21/16 incorporating synthetic threads
• CPC Class Codes: D10B 2509/08 Hernia repair mesh

Abstract

Cell cultures or tissue engineering supports, include at least a porous matrix based on a collagen sponge which defines first pores and a porous three-dimensional knit which defines second pores, the porous matrix filling the three-dimensional knit and all the first and second pores being at least partially interconnected with one another.

Claims

1. A cell culture or tissue engineering support, comprising:

a porous matrix based on a collagen sponge which defines first pores,

a porous three-dimensional knit which defines second pores,

said porous matrix filling said three-dimensional knit and all the first and second pores being at least partially interconnected with one another.

2. A support according to claim 1, wherein said porous matrix is bioresorbable.

3. A support according to claim 1, wherein said three-dimensional knit is bioresorbable.

4. A support according to claim 1, wherein in said collagen is a mixture of at least one collagen which undergoes slow bioresorption in vivo and at least one collagen which undergoes rapid bioresorption in vivo.

5. A support according to the preceding claim 4, wherein the collagen which undergoes slow bioresorption in vivo is selected from the group consisting of glutaraldehyde-crosslinked collagen, bifunctional or trifunctional glycidyl ethers, carbodiimides, acyl azides and mixtures thereof.

6. A support according to claim 4, wherein the collagen which undergoes rapid bioresorption in vivo is selected from the group consisting of oxidized collagen, glutaraldehyde-crosslinked collagen, bifunctional or trifunctional glycidyl ethers, carbodiimides, acyl azides, collagen crosslinked by UV irradiation or by heat treatment, and mixtures thereof.

7. A support according to claim 1, wherein the collagen forming said sponge is a mixture of oxidized collagen and glutaraldehyde-crosslinked collagen.

8. A support according to claim 1, wherein said three-dimensional knit consists of monofilament and/or multifilament yarns made of bioresorbable material which has an in vivo degradation time ranging from approximately 3 months to 2 years.

9. A support according to claim 8, wherein said bioresorbable material is selected from the group consisting of poly(lactic acid) (PLA), poly(glycolic acid) (PGA), oxidized cellulose, polycaprolactone (PCL), polydiaxanone (PDO), trimethylene carbonate (TMC), polyvinyl alcohol (PVA), polyhydroxyalkanoates (PHAs), polyamides, polyethers, copolymers thereof and mixtures thereof.

10. A support according to claim 1, wherein said second pores have an average diameter ranging from 1 to 5 mm.

11. A support according to claim 1, wherein said knit has a two-dimensional porosity of less than or equal to 20%.

12. A support according to claim 1, wherein said knit has a three-dimensional porosity of greater than or equal to 90%.

13. A support according to claim 1, wherein said three-dimensional knit has a thickness ranging from approximately 2 mm to 6 mm.

14. A support according to claim 1, wherein said three-dimensional knit comprises a first face and a second face, said first face and said second face being opposite and separated from one another by the thickness of said knit, said first face and said second face being connected to one another by a spacer made of monofilament yarns, multifilament yarns or a combination of monofilament yarns and multifilament yarns.

15. A support according to claim 14, wherein said spacer is made of monofilament yarns.

16. A support according to claim 14, wherein said first and second faces of the knit are made of monofilament yarns, multifilament yarns or a combination of monofilament and multifilament yarns.

17. A support according to claim 14, wherein the monofilament or multifilament yarns used to prepare the first and second faces and the spacer of the three-dimensional knit are chosen from yarns made of material which undergoes slow bioresorption, yarns made of material which undergoes rapid bioresorption, and mixtures thereof.

18. A support according to claim 17, wherein the yarns made of material which undergoes slow bioresorption are made of poly(lactic acid).

19. A support according to claim 17, wherein the yarns made of material which undergoes rapid bioresorption selected from the group consisting of poly(glycolic acid) yarns, oxidized cellulose yarns, poly(lactic acid) yarns partially degraded by a treatment such as repeat cycles of gamma-irradiation at doses of greater than or equal to 25 kGy, and mixtures thereof.

20. A support according to claim 15, wherein said monofilament yarns which make up said spacer consist of yarns made of material which undergoes slow bioresorption.

21. A support according to claim 20, wherein said monofilament yarns which make up said spacer are made of poly(lactic acid).

22. A support according to claim 17, wherein said first and second faces are made of a mixture of multifilament yarns made of material which undergoes slow bioresorption and of multifilament yarns made of material which undergoes rapid bioresorption.

23. A support according to claim 14, wherein said first and second faces are made of poly(lactic acid) multifilament yarns.

24. A support according to claim 1, wherein said knit is isoelastic.

25. A support according to claim 1, wherein said knit has a mechanical strength in the longitudinal direction, measured according to ISO standard 13934-1, ranging from 50 to 300 N.

26. A support according to claim 1, wherein said knit has a mechanical strength in the transverse direction, measured according to ISO standard 13934-1, ranging from 50 to 300 N.

27. A support according to claim 25, wherein said knit has a mechanical strength in the longitudinal direction, measured according to ISO standard 13934-1, ranging from 100 to 250 N.

28. A support according to claim 27, wherein said knit has a mechanical strength in the transverse direction, measured according to ISO standard 13934-1, ranging from 75 to 200 N.

29. A support according to claim 1, wherein said knit has an elongation at 50 N in the longitudinal direction, measured according to ISO standard 13934-1, ranging from 10% to 50%.

30. A support according to claim 1, wherein said knit has an elongation at 50 N in the transverse direction, measured according to ISO standard 13934-1, ranging from 10% to 50%.

31. A support according to claim 8, wherein at least a part of the yarns constituting said three-dimensional knit are coated with a bioresorbable coating.

32. A support according to claim 31, wherein said coating comprises collagen, chitosan, polysaccharides or mixtures thereof.

33. A support according to claim 31, wherein said coating is made of collagen.

34. A support according to claim 1, further comprising one or more active compounds selected from the group consisting of antiseptics, anti-inflammatories, growth factors, polysaccharides such as fucans, extracellular matrix proteins such as fibronectin, laminin, elastin, glycosaminoglycans or proteoglycans, and mixtures thereof.

35. A support according to claim 1, further comprising a bioresorbable film on at least one of its faces.

36. A support according to claim 35, wherein said film comprises at least one collagen.

37. A support according to claim 35, wherein said film comprises oxidized collagen, polyethylene glycol and glycerol.

38. A support according to claim 1, wherein the support is seeded with live cells.

39. A support according to claim 38, wherein the cells are selected from the group consisting of striated muscle cells, smooth muscle cells, endothelial cells, epithelial cells, mesothelial cells, fibroblasts, myofibroblasts, stem cells of striated muscle cells, stem cells of smooth muscle cells, stem cells of endothelial cells, stem cells of epithelial cells, stem cells of mesothelial cells, stem cells of fibroblasts, stem cells of myofibroblasts, and combinations thereof.

40. A support according to claim 38, wherein the cells are smooth muscle cells.

41. A support according to claim 38, wherein the cells are urothelial cells.

42. A support according to claim 35, wherein said collagen sponge is seeded with muscle cells and said bioresorbable film is seeded with endothelial or epithelial cells.

43. A support according to claim 35, wherein said collagen sponge is seeded with striated muscle cells and said bioresorbable film is seeded with mesothelial cells.

44. A support according to claim 35, wherein said collagen sponge is seeded with smooth muscle cells and said bioresorbable film is seeded with urothelial cells.