Soybean event 3560.4.3.5 and compositions and methods for the identification and/or detection thereof

• US 20090036308A1
• Filed: 05/30/2008
• Published: 02/05/2009
• Est. Priority Date: 06/28/2006
• Status: Active Grant

• Current Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.), Pioneer Hi Bred International Inc (Corteva, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.), Pioneer Hi Bred International Inc (Corteva, Inc.)
• Inventors: Hazel, Christine B., Hegstad, Jeffrey M., Xing, Aiqiu, Guida, Anthony D. JR., Li, Zhongsen, Vogt, Mark D.
• Granted Patent: US 7,968,770 B2
• Time in Patent Office: Days
• US Class Current: 504/206
• CPC Class Codes: C12N 15/8271 for stress resistance, e.g. heavy metal resistance
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8278 Sulfonylurea
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae

Abstract

Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided. Methods and compositions for increasing yield are also provided.

Claims

1. A method for improving yield in a soybean plant comprising treating said soybean plant with an effective amount of glyphosate, wherein said soybean plant comprises a 3560.4.3.5 soybean plant or a plant that is a progeny of a plant grown from seeds of ATCC Seed Deposit PTA-8287, and wherein said soybean plant further comprises a polynucleotide sequence encoding a polypeptide that imparts tolerance to glyphosate by a mode of action different than glyphosate N-acetyltransferase.

2. The method of claim 1, wherein said polynucleotide encodes a glyphosate-tolerant 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide.

3. The method of either claim 1, wherein the glyphosate is applied in a single treatment or in successive treatments.

4. The method of claim 1, wherein the glyphosate is a glyphosate derivative comprising a salt or a mixture of glyphosate salts selected from the group consisting of mono-isopropylammonium glyphosate, ammonium glyphosate, and sodium glyphosate.

5. The method of claim 1, wherein the glyphosate or derivative thereof is used in a formulation comprising an adjuvant selected from the group consisting of:

amines, ethoxylated alkyl amines, tallow amines, cocoamines, amine oxides, quaternary ammonium salts, ethoxylated quaternary ammonium salts, propoxylated quaternary ammonium salts, alkylpolyglycoside, alkylglycoside, glucose-esters, sucrose-esters, and ethoxylated polypropoxylated quaternary ammonium surfactants.

6. The method of claim 1 wherein said polynucleotide encodes a glyphosate oxidoreductase enzyme.

7. The method of claim 1 wherein said polynucleotide encodes a class II EPSPS enzyme.