APPARATUS AND METHOD FOR PERFORMING LIGAND BINDING ASSAYS ON MICROARRAYS IN MULTIWELL PLATES

• US 20090041633A1
• Filed: 05/14/2007
• Published: 02/12/2009
• Est. Priority Date: 05/14/2007
• Status: Abandoned Application

• Current Assignee: Maven Technologies, LLC
• Original Assignee: Maven Technologies, LLC
• Inventors: Travis, Jeffrey, Dultz, Shane C., Ralin, David
• Application Number:
  US11/748,023
• Publication Number:
  US 20090041633A1
• Time in Patent Office: Days
• US Class Current: 422/129
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates

Abstract

An apparatus and method for real time, label-free imaging and quantitation of binding events at an array of positions are provided. Total internal reflection from a planar side wall of a well of a multiwell plate is used to create an evanescent field in the plane of a pattern of ligands immobilized on the wall. Embodiments include imaging and multiple analyte detection and quantitation of a single wall of a single well as well as the simultaneous imaging and multiple analyte detection and quantitation of a number of wells.

Claims

1. Apparatus for performing binding ligand assays, comprising a planar transparent plate including at least one well therein, said at least one well having at least a first planar wall oriented out of the plane of said plate, the first planar wall having a plurality of ligands formed thereon in an array.