Composition comprising gas-filled microcapsules for ultrasound mediated delivery
First Claim
1. A composition comprising a bioactive agent and a plurality of gas-filled microcapsules having a polymeric and/or lipid shell, wherein said microcapsules have a resistance to a mechanical index (MI) of an applied ultrasound wave of at least 0.15 and said bioactive agent is substantially unbound to said shell.
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Accused Products
Abstract
Composition comprising gas filled microcapsules and a bioactive agent, useful for an ultrasound-mediated delivery of said bioactive agent. The microcapsules comprise a relatively stiff shell of polymeric or lipid material and have in particular a resistance to a mechanical index of at least 0.15, while the bioactive agent is substantially unbound to the shell of the microcapsules. The composition of the invention is particularly suitable for effectively delivering a genetic material into a cell, upon exposure of the composition to a level of acoustic pressure capable of destroying a portion of the microcapsules and releasing the gas contained therein.
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Citations
31 Claims
- 1. A composition comprising a bioactive agent and a plurality of gas-filled microcapsules having a polymeric and/or lipid shell, wherein said microcapsules have a resistance to a mechanical index (MI) of an applied ultrasound wave of at least 0.15 and said bioactive agent is substantially unbound to said shell.
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14. (canceled)
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15. A method for delivering a bioactive agent into a cell which comprises:
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administering a composition comprising a bioactive agent and a plurality of gas filled microcapsules having a polymeric and/or lipid shell to a body part comprising said cell in a patient in need thereof, said microcapsules having a resistance to a mechanical index of at least 0.15 and said bioactive agent being substantially unbound to said shell; and applying an ultrasound wave to said body part, said wave having an acoustic pressure capable of destroying a portion of said microcapsules, to effectively deliver said bioactive agent to said cell. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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- 23. A pharmaceutical kit for delivering a bioactive agent into a cell, comprising a suspension of a bioactive agent and suspension of a plurality of gas-filled microcapsules having a polymeric and/or lipid shell, said microcapsules having a resistance to a mechanical index (MI) of an applied ultrasound wave of at least 0.15 and said bioactive agent being substantially unbound to said shell.
- 24. A pharmaceutical kit for delivering a bioactive agent into a cell, comprising a bioactive agent and a plurality of microcapsules having a polymeric and/or lipid shell in form a dry powder form wherein, upon reconstitution with a physiologically acceptable carrier in the presence of a gas, said kit provides a suspension of said bioactive agent and of a plurality of gas-filled microcapsules, said microcapsules having a resistance to a mechanical index (MI) of an applied ultrasound wave of at least 0.15 and said bioactive agent being substantially unbound to said shell.
Specification