Mass Spectrometric Methods for Detecting Mutations in a Target Nucleic Acid
0 Assignments
0 Petitions
Accused Products
Abstract
Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided.
219 Citations
66 Claims
-
1-16. -16. (canceled)
-
17. A process for detecting the presence or absence of a target nucleic acid from a biological sample, comprising:
-
a) amplifying a nucleic acid from a biological sample; b) ionizing and volatilizing the product of step a); c) analyzing the product of step b) by mass spectrometry to determine an observed molecular mass of the product of step b); and d) comparing the observed molecular mass of the product of step b) to a calculated molecular mass of at least one nucleic acid having a known sequence, wherein the calculated molecular mass of the at least one nucleic acid having a known sequence is derived from the base composition of the at least one nucleic acid having a known sequence; whereby the presence or absence of the target nucleic acid is detected based on the comparison of (d). - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
-
-
43. A process for detecting a target nucleic acid from a biological sample, comprising:
-
a) amplifying a nucleic acid from a biological sample by PCR using at least one pair of primers, wherein said at least one pair of primers comprises a forward primer and a reverse primer; b) ionizing and volatilizing the product of step a); c) analyzing a first product of step b) by mass spectrometry to determine an observed molecular mass of the first product of step b), wherein said first product of step b) is the product extended from the forward primer; d) analyzing a second product of step b) by mass spectrometry to determine an observed molecular mass of the first product of step b), wherein said second product of step b) is the product extended from the reverse primer; e) comparing the observed molecular mass of the first product of step b) to a calculated molecular mass of a first nucleic acid having a known sequence, wherein the calculated molecular mass of the first nucleic acid having a known sequence is derived from the base composition of the first nucleic acid having a known sequence; f) comparing the observed molecular mass of the second product of step b) to a calculated molecular mass of a second nucleic acid having a known sequence, wherein the calculated molecular mass of the second nucleic acid having a known sequence is derived from the base composition of the second nucleic acid having a known sequence; whereby the presence or absence of the target nucleic acid is detected based on the comparisons of (e) and (f). - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66)
-
Specification