FLUIDIC CARTRIDGES FOR ELECTROCHEMICAL DETECTION OF DNA
First Claim
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1. A flow cell cartridge, said flow cell cartridge comprising:
- a housing forming a chamber, wherein the housing has a first opening adapted for the flow of a liquid and a second opening adapted for the flow of a gas, wherein said openings open to the inside of the chamber and movement of a gas out of said second opening is adapted to move a gas or a liquid into said first opening; and
an electrode array positioned inside said chamber such that a probe on the electrode array is exposed to the inside of the chamber, wherein the electrode array has an electrically conductive connection to a point external to the chamber, and wherein at least a portion of the electrode array has probe nucleic acid bound thereto.
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Abstract
A flow cell cartridge for the detection of differences in nucleic acid sequences is disclosed. The flow cell cartridge has an electrode array and two openings, in which one opening is for the entry and exit of sample, and the other opening is for the control of the entry and exit of sample through the exertion of negative and positive pressure. The entire flow cell cartridge can be moved from sample to sample to allow different samples to be drawn into the cartridge into contact with an electrochemical electrode array, thus allowing reactions to occur in the chamber itself.
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Citations
28 Claims
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1. A flow cell cartridge, said flow cell cartridge comprising:
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a housing forming a chamber, wherein the housing has a first opening adapted for the flow of a liquid and a second opening adapted for the flow of a gas, wherein said openings open to the inside of the chamber and movement of a gas out of said second opening is adapted to move a gas or a liquid into said first opening; and an electrode array positioned inside said chamber such that a probe on the electrode array is exposed to the inside of the chamber, wherein the electrode array has an electrically conductive connection to a point external to the chamber, and wherein at least a portion of the electrode array has probe nucleic acid bound thereto. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A flow cell cartridge, said flow cell cartridge comprising:
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a housing, said housing comprising
1) a front half and
2) a back half, wherein the front half when joined with the back half forms
3) a chamber, and wherein said housing has a first opening that is both liquid and gas permeable and a second opening that is gas permeable but is not significantly liquid permeable;at least a working electrode and a reference electrode, wherein said electrodes are in said chamber; and an electrical connection located on an external surface of the housing, wherein said electrical connection is in electrical contact with one or more of said electrodes. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of applying a sample to an electrode array, the method comprising:
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contacting a first opening of a flow cell cartridge (FCC) to a sample to be tested, wherein said FCC comprises a housing, said housing comprising a chamber, said chamber having a first opening and a second opening, and an electrode array contained within said chamber; and applying a negative pressure to the second opening for a period of time to move the sample through the first opening and into the chamber, thus promoting contact between the sample and the electrode array. - View Dependent Claims (23, 24, 25)
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26. (canceled)
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27. A method for performing an assay, comprising:
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providing a housing having a first opening, a second opening, a chamber interposed between said first and second opening, wherein said chamber includes at least one binding moiety for indicating a positive assay; providing at least first, second, and third wells containing liquids or reagents useful in performing an assay; positioning the first opening in liquid contact with the first well; moving liquid from the first well through the first opening and into the chamber by removing gas from the second opening; positioning the first opening in liquid contact with the second well; moving liquid from the second well through the first opening and into the chamber by removing gas from the second opening; positioning the first opening in liquid contact with the third well; moving liquid from the third well through the first opening and into the chamber by removing gas from the second opening; and ascertaining whether said binding moiety has participated in binding indicative of a positive assay.
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28-51. -51. (canceled)
Specification