PDX1 EXPRESSING ENDODERM
First Claim
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1. A method of producing PDX 1-positive endoderm cells, said method comprising:
- culturing a population of pluripotent human cells in the presence of exogenous TGFβ
superfamily growth factor;
removing exogenous TGFβ
superfamily growth factor from the cell population; and
continuing culturing the cell population in the presence of a retinoid, thereby producing multipotent PDX1-positive endoderm cells.
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Abstract
Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.
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18 Claims
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1. A method of producing PDX 1-positive endoderm cells, said method comprising:
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culturing a population of pluripotent human cells in the presence of exogenous TGFβ
superfamily growth factor;removing exogenous TGFβ
superfamily growth factor from the cell population; andcontinuing culturing the cell population in the presence of a retinoid, thereby producing multipotent PDX1-positive endoderm cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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- 12. An in vitro cell culture comprising human cells, wherein at least about 10% of said human cells are pancreatic-duodenal homeobox factor-1 (PDX1) positive endoderm cells.
Specification