Methods for Synthesizing a Collection of Partially Identical Polynucleotides
First Claim
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1. A method for synthesizing a collection of partially identical polynucleotides comprising:
- a) identifying a collection of unique, partially identical individual polynucleotide sequences to be synthesized;
b) analyzing the polynucleotide sequences with a phylogeny tree algorithm to make a phylogenetic tree;
c) identifying each individual branch group of the phylogenetic tree;
d) identifying each individual polynucleotide sequence in each individual branch group identified;
e) dividing each individual polynucleotide sequence in each branch group into smaller polynucleotide sequences;
f) identifying the smaller polynucleotide sequences common to all the individual polynucleotide sequences in each individual branch group identified if the branch group contains more than one individual polynucleotide sequence;
g) comparing the smaller polynucleotide sequences common to all the individual polynucleotide sequences in a first branch group with the smaller polynucleotide sequences from all other branch groups;
h) identifying the smaller polynucleotide sequences common to all branch groups;
i) identifying the smaller polynucleotide sequences common to only a subset of branch groups;
j) identifying the smaller polynucleotide sequences unique to each individual polynucleotide sequence in each branch group;
k) providing the smaller polynucleotide sequences of step h), step i), or step j) required to synthesize an individual polynucleotide sequence in a branch group;
l) synthesizing the individual partially identical polynucleotide sequence; and
m) repeating steps k) and l) until each individual polynucleotide sequence in the collection of partially identical polynucleotides is synthesized.
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Abstract
Methods for synthesizing a collection of partially identical polynucleotides are disclosed.
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4 Claims
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1. A method for synthesizing a collection of partially identical polynucleotides comprising:
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a) identifying a collection of unique, partially identical individual polynucleotide sequences to be synthesized; b) analyzing the polynucleotide sequences with a phylogeny tree algorithm to make a phylogenetic tree; c) identifying each individual branch group of the phylogenetic tree; d) identifying each individual polynucleotide sequence in each individual branch group identified; e) dividing each individual polynucleotide sequence in each branch group into smaller polynucleotide sequences; f) identifying the smaller polynucleotide sequences common to all the individual polynucleotide sequences in each individual branch group identified if the branch group contains more than one individual polynucleotide sequence; g) comparing the smaller polynucleotide sequences common to all the individual polynucleotide sequences in a first branch group with the smaller polynucleotide sequences from all other branch groups; h) identifying the smaller polynucleotide sequences common to all branch groups; i) identifying the smaller polynucleotide sequences common to only a subset of branch groups; j) identifying the smaller polynucleotide sequences unique to each individual polynucleotide sequence in each branch group; k) providing the smaller polynucleotide sequences of step h), step i), or step j) required to synthesize an individual polynucleotide sequence in a branch group; l) synthesizing the individual partially identical polynucleotide sequence; and m) repeating steps k) and l) until each individual polynucleotide sequence in the collection of partially identical polynucleotides is synthesized. - View Dependent Claims (2, 3, 4)
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Specification