NOVEL METHOD FOR SEQUENCE DETERMINATION USING NMR
First Claim
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1. A method of generating a list of possible sequences of an oligosaccharide in a heparin sample comprising:
- providing a heparin sample,defining a set of properties of an oligosaccharide in the heparin sample by performing nuclear magnetic resonance (NMR) spectroscopy and a second experimental method, wherein the NMR spectroscopy provides a measurement of a first type of disaccharide linkage and the second experimental method provides a measurement of a second type of disaccharide linkage;
constructing a list of possible oligosaccharides sequences based on the set of properties of the oligosaccharide, to thereby generate a list of possible sequences of the oligosaccharide in the heparin sample.
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Abstract
The invention relates to methods for analyzing polysaccharides. In particular, compositional and sequence information about the polysaccharides are derived. Some methods use NMR in conjunction with another experimental method, such as, capillary electrophoretic techniques for the analysis.
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38 Claims
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1. A method of generating a list of possible sequences of an oligosaccharide in a heparin sample comprising:
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providing a heparin sample, defining a set of properties of an oligosaccharide in the heparin sample by performing nuclear magnetic resonance (NMR) spectroscopy and a second experimental method, wherein the NMR spectroscopy provides a measurement of a first type of disaccharide linkage and the second experimental method provides a measurement of a second type of disaccharide linkage; constructing a list of possible oligosaccharides sequences based on the set of properties of the oligosaccharide, to thereby generate a list of possible sequences of the oligosaccharide in the heparin sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification