Molecular analysis of primary cells

• US 20090047656A1
• Filed: 03/13/2007
• Published: 02/19/2009
• Est. Priority Date: 03/13/2006
• Status: Abandoned Application

• Current Assignee: Veridex LLC (Johnson & Johnson)
• Original Assignee: Veridex LLC (Johnson & Johnson)
• Inventors: Burnett, Christine A., Choi, Chang H., Curtin, Kathleen M., Vener, Tatiana, Wang, Haiying, Baden, Jonathan F., Mazumder, Abhijit, Chowdary, Dondapati
• Application Number:
  US11/717,835
• Publication Number:
  US 20090047656A1
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/118 Prognosis of disease development
• CPC Class Codes: C12Q 2600/154 Methylation markers
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays
• CPC Class Codes: C12Q 2600/178 miRNA, siRNA or ncRNA

Abstract

The present invention provides a method of propagating cells of interest obtained from a biological specimen by a) enriching the cells under conditions that maintain sufficient cell viability; and b) propagating the cells under conditions effective to allow cell viability, proliferation and integrity.

Claims

19. A method of determining metastatic potential of a cell from a biological specimen comprising the steps of:

a) enriching cells from the specimen;

b) isolating nucleic acid and/or protein from the cells; and

c) analyzing the nucleic acid and/or protein to determine the presence, expression level or status of a Biomarker specific for metastatic potential.

20. A method of identifying mutations in hereditary diseases from a cell from a biological specimen comprising the steps of:

a) enriching cells from the specimen;

b) isolating nucleic acid and/or protein from the cells; and

c) analyzing the nucleic acid and/or protein to determine the presence, expression level or status of a Biomarker specific for a hereditary disease.

21. A method of preserving genetic material from a cell from a biological specimen comprising the steps of:

a) enriching cells from the specimen;

b) isolating nucleic acid and/or protein from the cells; and

c) preserving the nucleic acid and/or protein.

22. A method of making a tumor cell vaccine comprising the steps of

a) obtaining a biological specimen

b) enriching cells from the specimen;

c) isolating nucleic acid and/or protein from the cells; and

d) using the nucleic acid and/or protein to formulate the vaccine.

24-1. A kit comprising biomarker detection agents for performing the method according to claim 1.