REAGENTS, KITS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES BY ENERGY TRANSFER FROM AN ACTIVATED CHEMILUMINESCENT SUBSTRATE TO AN ENERGY ACCEPTOR DYE
First Claim
Patent Images
1. An article of manufacture comprising;
- a support comprising a surface;
a chemiluminescent enhancing material on the surface of the support;
an energy acceptor dye on the surface of the support; and
one or more biomolecular probes on the surface of the support.
8 Assignments
0 Petitions
Accused Products
Abstract
Reagents, kits and methods for detecting biological molecules by energy transfer from an activated chemiluminescent substrate to an energy acceptor dye such as a J-aggregated dye are described.
19 Citations
67 Claims
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1. An article of manufacture comprising;
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a support comprising a surface; a chemiluminescent enhancing material on the surface of the support; an energy acceptor dye on the surface of the support; and one or more biomolecular probes on the surface of the support. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 32, 63, 64, 65, 66, 67)
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22-31. -31. (canceled)
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33-40. -40. (canceled)
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41. A kit for detecting analyte in a sample comprising:
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an article of manufacture comprising a support having a surface, an energy acceptor dye on the surface of the support, and one or more biomolecular probes on the surface of the support, wherein the one or more biomolecular probes is capable of binding to the analyte or, when the analyte is present, which binds to the analyte; and a chemiluminescent substrate.
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42-51. -51. (canceled)
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52. A method for detecting multiple analytes in a sample comprising:
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contacting the sample with a first article of manufacture comprising;
a support having a surface;
a first chemiluminescent enhancing material on the surface of the support;
a first energy acceptor dye on the surface of the support; and
a first biomolecular probe on the surface of the support, wherein the first biomolecular probe is capable of binding to a first analyte;contacting the sample with a second article of manufacture comprising;
a support having a surface;
a second chemiluminescent enhancing material on the surface of the support;
a second energy acceptor dye on the surface of the support; and
a second biomolecular probe on the surface of the support, wherein the second biomolecular probe is capable of binding to a second analyte;allowing first analyte in the sample to bind to the first probe, wherein;
(a) the first analyte is a first enzyme;
(b) the first analyte is labeled with a first enzyme;
(c) the support surface of the first article of manufacture is contacted with a biomolecule which is labeled with a first enzyme and which binds to the first analyte on the support surface;
or (d) the first analyte is unlabeled and first analyte labeled with a first enzyme is added to the sample to allow the enzyme-labeled first analyte in the sample to compete with the unlabeled first analyte for binding to the first biomolecular probe of the first article of manufacture;allowing second analyte in the sample to bind to the second probe wherein;
(a) the second analyte is a second enzyme;
(b) the second analyte is labeled with a second enzyme;
(c) the second support surface of the second article of manufacture is contacted with a biomolecule which is labeled with a second enzyme and which binds to the second analyte on the support surface;
or (d) the second analyte is unlabeled and second analyte labeled with a second enzyme is added to the sample to allow the enzyme-labeled second analyte in the sample to compete with the unlabeled second analyte for binding to the second biomolecular probe of the second article of manufacture;contacting the first article of manufacture with a first chemiluminescent substrate which is activated by the first enzyme, wherein the activated first chemiluminescent substrate excites the first energy acceptor dye resulting in emissions therefrom and contacting the second article of manufacture with a second chemiluminescent substrate which is activated by the second enzyme, wherein the activated second chemiluminescent substrate excites the second energy acceptor dye resulting in emissions therefrom; and detecting emissions from the first energy acceptor dye and detecting emissions from the second energy acceptor dye, wherein the emissions from the first energy acceptor dye are distinguishable from those of the second energy acceptor dye.
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53-57. -57. (canceled)
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58. A kit for detecting multiple analytes in a sample comprising:
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a first article of manufacture comprising;
a first support having a surface;
a first chemiluminescent enhancing material on the surface of the support;
a first energy acceptor dye on the surface of the support; and
a first biomolecular probe on the surface of the support, wherein the first biomolecular probe comprises a first probe capable of binding to a first analyte or, when the first analyte is present, which binds to the first analyte;a second article of manufacture comprising;
a support having a surface;
a second chemiluminescent enhancing material on the surface of the support;
a second energy acceptor dye on the surface of the support; and
a second biomolecular probe on the surface of the support, wherein the second biomolecular probe comprises a first probe capable of binding to a second analyte or, when the second analyte is present, which binds to the second analyte;a first chemiluminescent substrate, wherein the activated first chemiluminescent substrate excites the first energy acceptor dye resulting in emissions therefrom; and a second chemiluminescent substrate, wherein the activated second chemiluminescent substrate excites the second energy acceptor dye resulting in emissions therefrom, wherein the emissions from the first energy acceptor dye are distinguishable from those of the second energy acceptor dye.
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59-62. -62. (canceled)
Specification