PROCESS FOR STARCH LIQUEFACTION AND FERMENTATION
First Claim
1. A method of liquefaction comprising the steps of:
- a) milling plant material comprising between about 0.1% to about 100% admix of a transgenic plant expressing at least one starch-digesting enzyme;
b) combining the milled plant material with a liquid to form a mash; and
c) hydrolyzing starch in the mash in a continuous flow of the mash without the use of a slurry tank or a liquefaction tank.
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Accused Products
Abstract
The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.
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Citations
12 Claims
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1. A method of liquefaction comprising the steps of:
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a) milling plant material comprising between about 0.1% to about 100% admix of a transgenic plant expressing at least one starch-digesting enzyme; b) combining the milled plant material with a liquid to form a mash; and c) hydrolyzing starch in the mash in a continuous flow of the mash without the use of a slurry tank or a liquefaction tank. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method of liquefaction comprising the steps of,
a) milling plant material comprising between about 8% to about 50% admix of a transgenic plant expressing an alpha-amylase; -
b) combining the milled plant material with a liquid to form a mash comprising at least 37% solids; c) heating the mash to a temperature that does not exceed 90 degrees C.; d) adjusting the pH of the mash to between about 3.8 to about 5.0; and e) hydrolyzing the starch in the mash in a continuous flow of the mash in one or more pipes or tubes without the use of a slurry tank or a liquefaction tank.
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Specification