Site specific protein modification
First Claim
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1. A method of site specifically labeling a protein comprising:
- i) constructing a fusion protein comprising;
(a) a protein comprising at least a single unprotected and uniquely reactive amino acid, and(b) at least one domain comprising one or more uniquely protected reactive amino acids,ii) reacting said unprotected amino acid of protein (a) with a first modifying agent so that a covalent linkage between said unprotected amino acid and said first modifying agent is formed, andiii) deprotecting said one or more reactive amino acids of domain (b) and reacting said one or more deprotected reactive amino acids with a second modifying agent so that a covalent linkage between said one or more deprotected reactive amino acids and said second modifying agent is formed.
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Abstract
The present invention relates, in general, to protein modifications and, in particular, to a method of effecting site-specific labeling of proteins with covalently coupled reporter groups. The invention further relates to a method of effecting orientation-specific immobilization of proteins on a solid surface. The invention also relates to products produced by such methods.
12 Citations
1 Claim
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1. A method of site specifically labeling a protein comprising:
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i) constructing a fusion protein comprising; (a) a protein comprising at least a single unprotected and uniquely reactive amino acid, and (b) at least one domain comprising one or more uniquely protected reactive amino acids, ii) reacting said unprotected amino acid of protein (a) with a first modifying agent so that a covalent linkage between said unprotected amino acid and said first modifying agent is formed, and iii) deprotecting said one or more reactive amino acids of domain (b) and reacting said one or more deprotected reactive amino acids with a second modifying agent so that a covalent linkage between said one or more deprotected reactive amino acids and said second modifying agent is formed.
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Specification
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Current AssigneeDuke University
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Original AssigneeDuke University
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InventorsConrad, David W., Smith, James J., Hellinga, Homme W.
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Application NumberUS11/987,006Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current530/335CPC Class CodesC07K 1/13 Labelling of peptides
