Homogeneous Chemiluminescent Immunoassay for Analysis of Iron Metalloproteins
First Claim
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1. A method of detecting an iron metalloprotein in a test sample, the method comprising the steps of:
- a) adding an acridinium-9-carboxamide-antibody conjugate to the test sample, wherein the antibody specifically binds the iron metalloprotein;
b) generating in or providing to the test sample a source of hydrogen peroxide before or after the addition of an acridinium-9-carboxamide-antibody conjugate;
c) adding a basic solution to the test sample to generate a light signal; and
d) quantifying the light generated to detect the iron metalloprotein.
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Abstract
The present invention relates to assays and kits for detecting or quantifying iron metalloprotein in test samples.
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Citations
17 Claims
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1. A method of detecting an iron metalloprotein in a test sample, the method comprising the steps of:
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a) adding an acridinium-9-carboxamide-antibody conjugate to the test sample, wherein the antibody specifically binds the iron metalloprotein; b) generating in or providing to the test sample a source of hydrogen peroxide before or after the addition of an acridinium-9-carboxamide-antibody conjugate; c) adding a basic solution to the test sample to generate a light signal; and d) quantifying the light generated to detect the iron metalloprotein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. The method of claim 9, wherein the standard curve is generated from solutions of an iron metalloprotein of a known concentration.
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10. A kit for use in detecting an iron metalloprotein in a test sample, the kit comprising:
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a. at least one acridinium-9-carboxamide; b. at least one antibody that specifically binds the iron metalloprotein; c. at least one basic solution; d. a source of hydrogen peroxide; and e. instructions for detecting an iron metalloprotein in a test sample. - View Dependent Claims (11, 12)
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13. A kit for use in detecting an iron metalloprotein in a test sample, the kit comprising:
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a. at least one acridinium-9-carboxamide; b. at least one antibody that specifically binds the iron metalloprotein; c. at least one basic solution; d. a means of generating hydrogen peroxide in situ in the test sample; and e. instructions for detecting an iron metalloprotein in a test sample. - View Dependent Claims (14, 15, 16, 17)
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Specification