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Homogeneous Chemiluminescent Immunoassay for Analysis of Iron Metalloproteins

  • US 20090053736A1
  • Filed: 08/21/2007
  • Published: 02/26/2009
  • Est. Priority Date: 08/21/2007
  • Status: Abandoned Application
First Claim
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1. A method of detecting an iron metalloprotein in a test sample, the method comprising the steps of:

  • a) adding an acridinium-9-carboxamide-antibody conjugate to the test sample, wherein the antibody specifically binds the iron metalloprotein;

    b) generating in or providing to the test sample a source of hydrogen peroxide before or after the addition of an acridinium-9-carboxamide-antibody conjugate;

    c) adding a basic solution to the test sample to generate a light signal; and

    d) quantifying the light generated to detect the iron metalloprotein.

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