Process For Concentrated Biomass Saccharification
First Claim
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1. A process for producing high-sugar content hydrolysate from biomass comprising:
- a) providing a portion of reaction components in a vertical stirred tank reactor having a particle size reduction mechanism, said reaction components comprising;
i) a portion of a mixable pretreated biomass slurry; and
ii) a portion of a first saccharification enzyme consortium comprising at least one enzyme capable of hydrolyzing cellulose;
b) reacting said slurry and enzymes under suitable conditions;
c) applying the particle size reduction mechanism;
d) adding an additional portion of pretreated biomass producing a higher solids biomass slurry;
e) optionally adding an additional portion of a saccharification enzyme consortium;
f) reacting said higher solids biomass slurry under suitable conditions; and
g) optionally repeating one or more steps (c), (d), (e), and (f) one or more times,whereby a high sugar content hydrolysate is produced and wherein the yield stress of the slurry is maintained at less than 30 Pa.
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Abstract
Processes for saccharification of pretreated biomass to obtain high concentrations of fermentable sugars are provided. Specifically, a process was developed that uses a fed batch approach with particle size reduction to provide a high dry weight of biomass content enzymatic saccharification reaction, which produces a high sugars concentration hydrolysate, using a low cost reactor system.
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27 Claims
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1. A process for producing high-sugar content hydrolysate from biomass comprising:
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a) providing a portion of reaction components in a vertical stirred tank reactor having a particle size reduction mechanism, said reaction components comprising; i) a portion of a mixable pretreated biomass slurry; and ii) a portion of a first saccharification enzyme consortium comprising at least one enzyme capable of hydrolyzing cellulose; b) reacting said slurry and enzymes under suitable conditions; c) applying the particle size reduction mechanism; d) adding an additional portion of pretreated biomass producing a higher solids biomass slurry; e) optionally adding an additional portion of a saccharification enzyme consortium; f) reacting said higher solids biomass slurry under suitable conditions; and g) optionally repeating one or more steps (c), (d), (e), and (f) one or more times, whereby a high sugar content hydrolysate is produced and wherein the yield stress of the slurry is maintained at less than 30 Pa. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification