TRAPPING MAGNETIC SORTING SYSTEM FOR TARGET SPECIES

• US 20090053799A1
• Filed: 08/22/2008
• Published: 02/26/2009
• Est. Priority Date: 08/23/2007
• Status: Abandoned Application

• Current Assignee: Cynvenio Biosystems Inc.
• Original Assignee: Cynvenio Biosystems, LLC
• Inventors: Pagano, Paul, Soh, Hyongsok T., Chang-Yen, David A., Zhang, Yanting, Darabi, Jafar
• Application Number:
  US12/197,169
• Publication Number:
  US 20090053799A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B03C 1/01 by addition of magnetic adjuvants
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 2201/26 for use in medical applications

Abstract

A system for sorting and trapping magnetic target species includes a microfluidic trapping module designed to receive and then temporarily hold magnetic particles in place within the module. The magnetic particles flowing into the module are trapped there while the other sample components (non-magnetic) continuously flow through and out of the station, thereby separating and concentrating the species captured on the magnetic particles. The magnetic particles and/or their payloads may be released and separately collected at an outlet after the sample passes through the trapping module.

Claims

1. A microfluidic sorting device for sorting a sample into substantially target and substantially non-target species, the device comprising:

(a) a trapping module comprising a channel having two opposing walls, a magnetic field gradient generating structure on only one of the opposing walls for exerting a magnetic force on a sample to capture, at least temporarily, magnetic particles in the sample; and

(b) a pre-processing and/or a post-processing station integrated on the microfluidic sorting device with trapping module.

2. The microfluidic sorting device of claim 1, wherein the opposing wall having the magnetic field gradient generating structure is on an opaque wall.

3. The microfluidic sorting device of claim 1, further comprising an external source on one side of the trapping module for generating a magnetic field.

4. The microfluidic sorting device of claim 3, wherein the external source is located on the same side of the trapping module as the magnetic field gradient generating structure.

5. The microfluidic sorting device of claims 3, further comprising a mechanism for moving the external source of the magnetic field with respect to the trapping module.

6. The microfluidic sorting device of claims 3, wherein the external source of the magnetic field comprises one or more permanent magnets.

7. The microfluidic sorting device of claim 6, wherein the permanent magnets alternate in polarity.

8. The microfluidic sorting device of claim 3, wherein the external source of the magnetic field comprises one or more electromagnets.

9. The microfluidic sorting device of claim 1, wherein the magnetic field gradient generating structure comprises a ferromagnetic structure laid out in a pattern on its opposing wall.

10. The microfluidic sorting device of claim 9, wherein the pattern is selected from the group consisting of parallel lines, an orthogonal grid, a rectangular array of regular or irregular geometric shapes, and combinations thereof.

11. The microfluidic sorting device of claim 1, wherein the magnetic field gradient generating structure comprises a random array of ferromagnetic structures.

12. The microfluidic sorting device of claims 9, wherein the ferromagnetic structure material is nickel, vanadium permedur, or permalloy.

13. The microfluidic sorting device of claim 1, wherein the channel has a larger depth in the trapping region.

14. The microfluidic sorting device of claim 1, wherein the trapping module comprises a staged trapping system with two or more trapping regions.

15. The microfluidic sorting device of claim 1, further comprising a second parallel, independent trapping module;

and a common buffer manifold connecting the trapping modules.

16. The microfluidic sorting device of claim 1, wherein the pre-processing station comprises a labeling station for labeling a species in the sample with magnetic particles having an affinity for the labeled species.

17. The microfluidic sorting device of claim 1, wherein the post-processing station comprises a detection station for detecting the target species.

18. The microfluidic sorting device of claim 1, wherein at least one of the pre-processing station or the post-processing station comprise (a) an enrichment module for increasing a concentration of a target species in a sample passing through the sorting device, (b) a reaction module, (c) a detection module, and (d) a lysis module for lysing cells, disrupting viral protein coats, or otherwise releasing components of small living systems.

19. The microfluidic sorting device of claim 1, wherein the trapping module is designed or configured to perform an operation selected from the group consisting of (1) genomic analysis;

(2) a detection and/or amplification scheme for DNA or RNA oligomers;

(3) gene expression;

(4) enzymatic activity assays;

(5) receptor binding assays; and

(6) ELISA assays.

20. A method for sorting a sample in a microfluidic sorting device that includes a trapping module and a magnetic field gradient generating structure on only one of two opposing walls of the trapping module, the method comprising:

flowing a sample into the trapping module, said sample comprising a plurality magnetic particles with molecular recognition elements thereon, a target species, and a non-target species;

generating a magnetic field gradient in the trapping module by exerting an external magnetic field from only one side of the module to the magnetic field gradient generating structure; and

trapping magnetic particles in the trapping module proximate to the magnetic field gradient generating structure on one wall of the trapping module.

21. The method for sorting a sample of claim 20, further comprising:

flowing a buffer concurrently with the sample into the trapping module.

22. The method for sorting a sample of claims 21, wherein the buffer is continuously flowed into the trapping module during every operation.

23. The method for sorting a sample of claims 20, further comprising:

moving an external source of the magnetic field with respect to the trapping module while the magnetic particles flow through the module in the fluid medium to thereby trap magnetic particles in a substantially uniform fashion.

24. The method for sorting a sample of claims 20, further comprising:

releasing the magnetic particles from one section of the magnetic field gradient generating structure to release any trapped non-magnetic particles; and

trapping the magnetic particles in another section of the magnetic field gradient generating structure.

25. The method for sorting a sample of claim 20, further comprising:

labeling the target species in the sample with magnetic particles having an affinity for the target species.

26. The method for sorting a sample of claim 20, further comprising:

labeling the non-target species in the sample with magnetic particles having an affinity for the non-target species.

27. The method for sorting a sample of claim 20, further comprising:

detecting the target species in a microarray.

28. The method for sorting a sample of claim 20, further comprising:

lysing the target species.

29. The method for sorting a sample of claim 20, further comprising:

reacting the target species to amplify, sequence, hybridize, label, crosslink or culture the target species.

30. The method for sorting a sample of claim 20, further comprising:

imaging the trapped target species.

31. A microfluidic sorting device for sorting a sample into substantially target and substantially non-target species, the device comprising:

(a) a trapping module comprising a channel having two opposing walls and no magnetic field gradient generating structure;

(b) a pre-processing and/or a post-processing station integrated on the microfluidic sorting device with trapping module;

(c) an external source on one side of the trapping module for generating a magnetic field; and

(d) a mechanism to vary the magnetic field over time.