Thermal Reaction Device and Method for Using the Same
First Claim
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1. A method for performing an analysis of a sample comprising the steps of:
- providing a microfluidic device having a channel dimension between 0.1 and 1000 μ
m, and having at least one sample channel, wherein the sample channel is able to be partitioned into a plurality of separate chambers by one or more valves, said valves being formed in said microfluidic device such that a deflectable membrane(s) can be deflected into said sample channel to partition said sample channel into one or more separate chambers, introducing a sample having at least one amplifiable target therein into said sample chamber, actuating said valves to form said separate chambers so that said at least one amplifiable target is isolated into at least one chamber, conducting an amplification step or steps, detecting whether amplification occurred in one or more chambers to determine the quantity or presence of the amplifiable target within the sample.
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Abstract
An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
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Citations
1 Claim
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1. A method for performing an analysis of a sample comprising the steps of:
- providing a microfluidic device having a channel dimension between 0.1 and 1000 μ
m, and having at least one sample channel, wherein the sample channel is able to be partitioned into a plurality of separate chambers by one or more valves, said valves being formed in said microfluidic device such that a deflectable membrane(s) can be deflected into said sample channel to partition said sample channel into one or more separate chambers, introducing a sample having at least one amplifiable target therein into said sample chamber, actuating said valves to form said separate chambers so that said at least one amplifiable target is isolated into at least one chamber, conducting an amplification step or steps, detecting whether amplification occurred in one or more chambers to determine the quantity or presence of the amplifiable target within the sample.
- providing a microfluidic device having a channel dimension between 0.1 and 1000 μ
Specification