ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC FEEDSTOCKS USING ACCESSORY ENZYMES
First Claim
1. A process for converting a pretreated lignocellulosic feedstock to soluble sugars comprising enzymatically hydrolyzing the pretreated lignocellulosic feedstock with a cellulase enzyme mixture, the cellulase enzyme mixture comprising EG4, Swollenin and Cip1 accessory enzymes, whereinthe EG4 accessory enzyme is present at a fractional concentration (fEG4) of about 0.09-0.91 (w/w),the Swollenin accessory enzyme is present at a fractional concentration (fSwo1) of about 0.09 to about 0.91 (w/w), andthe Cip1 accessory enzyme is present at a fractional concentration (fCip1) of 0 to about 0.42 (w/w)measured relative to all accessory enzymes present in said enzyme mixture.
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Abstract
Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.
33 Citations
44 Claims
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1. A process for converting a pretreated lignocellulosic feedstock to soluble sugars comprising enzymatically hydrolyzing the pretreated lignocellulosic feedstock with a cellulase enzyme mixture, the cellulase enzyme mixture comprising EG4, Swollenin and Cip1 accessory enzymes, wherein
the EG4 accessory enzyme is present at a fractional concentration (fEG4) of about 0.09-0.91 (w/w), the Swollenin accessory enzyme is present at a fractional concentration (fSwo1) of about 0.09 to about 0.91 (w/w), and the Cip1 accessory enzyme is present at a fractional concentration (fCip1) of 0 to about 0.42 (w/w) measured relative to all accessory enzymes present in said enzyme mixture.
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25. A cellulase enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars, the cellulase enzyme mixture comprising EG4, Swollenin and Cip1 accessory enzymes, wherein
the EG4 accessory enzyme is present at a fractional concentration (fEG4) of about 0.09 to about 0.91 (w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture, the Swollenin accessory enzyme is present at a fractional concentration (fSwo1) of about 0.09 to about 0.91 (w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture, and the Cip1 accessory enzyme being present at a fractional concentration (fCip1) of 0 to about 0.42 measured relative to all accessory enzymes present in the cellulase enzyme mixture.
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39. A process for converting a pretreated lignocellulosic feedstock to soluble sugars comprising enzymatically hydrolyzing the pretreated lignocellulosic feedstock with a cellulase enzyme mixture, the cellulase enzyme mixture comprising EG4, Swollenin and Cip1 accessory enzymes, wherein
the EG4 accessory enzyme is present at a fractional concentration (fEG4) of about 0.25-0.83 (w/w), said Swollenin accessory enzyme is present at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and said Cip1 accessory enzyme is present at a fractional concentration (fCip1) of 0 to about 0.33 w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture.
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40. A cellulase enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars, the cellulase enzyme mixture comprising EG4, Swollenin and Cip1 accessory enzymes, wherein
the EG4 accessory enzyme is present at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture, the Swollenin accessory enzyme is present at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture, and the Cip1 accessory enzyme being present at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the cellulase enzyme mixture.
- 41. A method for producing a cellulase enzyme mixture comprising EG4, Swollenin and Cip1 accessory enzymes comprising, expressing the cellulase enzyme mixture from one or more microorganisms so as to produce the EG4 at a fractional concentration (fEG4) of about 0.25-0.83 (w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture, the Swollenin enzyme at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture and the Cip1 enzyme at a fractional concentration (fCip1) of 0 to about 0.33 (w/w) measured relative to all accessory enzymes present in the cellulase enzyme mixture.
Specification